Cytotoxicity Calcium Hydroxide Nanoparticulated Material in Human Dental Pulp Cells
Objectives: Synthesize and to characterize calcium hydroxide (Ca(OH)2) nanoparticulated (CaOH-NP) and compare to the cytotoxicity of Ca(OH)2 past (Ultracal), MTA-White and CaOH-NP in human dental pulp cells (HDPCs) stimulated with lipopolysaccharide (LPS). Methods: The nanoparticles were synthesised by coprecipitation method. The physical properties were investigated through X-Rays Diffraction, spectroscopy Raman, Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). HDPCs LPS stimulated were placed in contact with different dilutions of culture media previously exposed to such materials (extracts). The groups were tested for cell viability by MTT formazan and Alamar Blue assays, production of nitric oxide (NO) by Griess method and production of reactive oxygen species (ROS) by means of a fluorescent oxidant-sensing probe 2’.7’-dichlorodihydrofluorescein diacetate (DCFH-DA. The results were statistically analyzed by one-way ANOVA, Tukey and Dunnett tests (α=0.05). Results: The results showed that the cell viability remained above 50% in all materials independent of the dilution tested in both the MTT formazan and Alamar Blue tests. The NO production in UC and CaOH-NP groups were similar with negative control (DMEM) (p > 0.05) and positive control (LPS) (p > 0.05), in all dilutions. MTA showed a reduction in NO production at dilutions of 1: 4 to 1:32 compared to the positive control group (p < 0.05). All tested materials reduced ROS production by HDPCs compared to the positive control group (p < 0.05) and similar to negative control (p > 0.05), except for the UC group at concentrations of 1: 1, which presented higher values compared to the negative control group (p < 0.05). Conclusions: The present results showed that MTA, UC and CaOH-NP were biocompatible materials in relation to cell viability, but MTA presented results closer to DMEM. All materials tested modulated ROS production and only the MTA in the higher dilutions inhibited NO production.
Division: IADR/AADR/CADR General Session
Meeting:2019 IADR/AADR/CADR General Session (Vancouver, BC, Canada) Location: Vancouver, BC, Canada
Year: 2019 Final Presentation ID:0710 Abstract Category|Abstract Category(s):Dental Materials 5: Biocompatibility, Bioengineering and Biologic Effects of Materials
Authors
Oliveira, Lilian
( Federal University of Uberlândia
, Uberlândia
, Brazil
)
De Souza, Gabriela
( Federal University of Uberlândia
, Uberlândia
, MG
, Brazil
)
Silva, Anielle
( Federal University of Alagoas
, Alagoas
, Brazil
)
Dantas, Noelio
( Federal University of Alagoas
, Alagoas
, Brazil
)
Turrioni, Ana Paula
( Federal University of Uberlândia
, Uberlândia
, Brazil
)
Moura, Camilla
( Federal University of Uberlândia
, Uberlândia
, MG
, Brazil
)