Molecular Analysis of Bacteria Associated with Apical Periodontitis

Objectives: The main objective of the present study was to study the prevalence and relationship of F.Nucleatum, P.Gingivalis, T.Denticola, T. Forsythia and P.Endodontalis from different forms of apical periodontitis lesions.

Methods: Adult patients with one clinically and radiographically documented periapical lesion were included. Samples were collected by means of a size 15 H-type file and two sterile paper points from 80cases of primary endodontic infections using a septic technique, the prevalence and quantity of bacteria was determined using quantitative real time PCR.
Results: F.nucleatum was found to be the most prevalent bacteria (60%) followed by T.denticola (46%), P.endodontalis (31%), P.gingivalis (24%) and T. forsythia (17%). A significant association was found between F,Nucleatum and T,denticola with symptomatic apical periodontitis (p< 0.05).
Conclusions: P.gingivalis has the highest bacterial mean count among the other bacteria investigated. F.Nucleatum is the most prevalent bacteria among the bacteria under study.