Objectives: Traumatic dental injuries (TDI) often happens in early childhood, the examination and diagnosis methods are clinical and radiograph. Another methodology that has been used is molecular biology. A fluid that has been studied in molecular biology is gingival crevicular fluid (GCF), which is appropriate to evaluate the relation between periodontal tissues and pulp, and its compounds will depend on either the health or inflammation level of tissues. This study aimed to identify the protein and peptides in GCF of primary tooth with TDI to find a proteomic signature to contribute in the diagnosis of pulp and periodontal conditions. Methods: The sample was composed for 8 children with TDI, ranging 4 to 6 years. GCF samples were collected in gingival sulcus of both the 12 traumatized teeth and the 8 primary first molar, divided into 5 groups: Molar group (deciduous first molar) (G1), No alteration (G2), Pulp Canal Obliteration (G3), Repair-Related Resorption (G4) and Pulp Necrosis (G5). GCF proteins were subjected to liquid chromatography electrospray ionization mass spectrometry for identification and characterization. Results: 436 single proteins/peptides were found in this study. 43 (9.86%) were in G2, 92 (21.10%) in G3 group, 23 (5.28%) in G4 group, 16 (3.67%) in G5group, and finally, 114 (26.15%) in molar group. Proteins functions related to the specific groups: G1 played role in homeostasis process. G2 group: nervous tissue, odontoblast differentiation. G3 group: senescence and mutation are associate with skeletal deformation. G4 group: response to hypoxia, fibroblast transformation, and osteoblast differentiation; stem cell differentiation releasing calcium ion into cytosol. G5 group: development of neuronal tissue; neurotransmitter release, cell division and death; play a role in conducting axon; mitotic process; DNA repair; immune defense. Conclusions: In conclusion, the protein profile of TDI could assist in the prognostic of dental pulp and periodontal conditions.
Division: IADR/AADR/CADR General Session
Meeting:2019 IADR/AADR/CADR General Session (Vancouver, BC, Canada) Location: Vancouver, BC, Canada
Year: 2019 Final Presentation ID:3318 Abstract Category|Abstract Category(s):Pediatric Oral Health Research
Authors
Machado, Maria
( University of Sao Paulo
, Bauru
, Sao Paulo
, Brazil
)
Xiao, Yhizhi
( University of Western Ontario
, London
, Ontario
, Canada
)
Siqueira, Walter
( University of Western Ontario
, London
, Ontario
, Canada
)
Jorge, Paula
( University of Sao Paulo
, Bauru
, Sao Paulo
, Brazil
)
Cardoso, Mariane
( UFSC
, Florianópolis
, Santa Catarina
, Brazil
)
Lourenço Neto, Natalino
( University of Sao Paulo
, Bauru
, Sao Paulo
, Brazil
)
Santos, Carlos
( Bauru School of Dentistry/ University of São Paulo
, Bauru
, São Paulo
, Brazil
)
Bringel, Mayara
( University of Sao Paulo
, Bauru
, Sao Paulo
, Brazil
)
Colombini-ishikiriama, Bella
( Bauru School of Dentistry/ University of São Paulo
, Bauru
, São Paulo
, Brazil
)
Moffa, Eduardo
( UNIFAE
, São João da Boa Vista
, São Paulo
, Brazil
)
Oliveira, Thais
( University of Sao Paulo
, Bauru
, Sao Paulo
, Brazil
)
Support Funding Agency/Grant Number: CNPq 203014/2015-4 and 401390/2014-4
Financial Interest Disclosure: NONE