IADR Abstract Archives
2D Nanomaterials Functionalized 3D Nanofibrous Scaffolds for Bone Regeneration
Objectives: Developing a biomaterial that can promote osteoblastic differentiation, thereby reducing the needs of exogenous osteogenic factors, has been a significant and long-term technical hurdle. In this study, we report an innovative 3D nanocomposite scaffold to address the common limitations with current polymeric scaffolds, including: 1) low mechanical properties, 2) poor osteoconductivity, and 3) a limited drug delivery capacity.
Methods: By using thermally induced phase separation (TIPS) method together with particle leaching technique (TIPS&P), a synthetic 2D nanosilicate (NS)-functionalized 3D gelatin nanofirous scaffold (GF) was developed to morphologically and chemically mimic native bone-matrix. The morphology and microstructure of 2D and 3D GF scaffolds were studied by using Quanta standard Environmental SEM (FEI, USA). The osteogenic differentiation of hMSCs on scaffolds were studied by testing the ALP activity, calcium content, and osteogenic gene expression. In addition to in vitro study, the effects of composite scaffolds on rhBMP2-induced bone formation were investigated by using an ectopic bone formation model in C57BL/6NHsd male mice (n=6).
Results: In addition to the significantly higher mechanical strength, the composite scaffolds (GF/NS) demonstrated significantly stronger ability to promote the osteogenic differentiation of hMSCs in vitro compared to the neat scaffold (GF). Our data further revealed that this intriguing pro-osteoblastic functionality was largely because of the unique features of the biodegradable nanoclay, particularly, the strong binding ability to pro-osteoblastic factors (e.g., BMP2 and small molecules) to achieve sustained release in addition to the intrinsic osteoinductivity by virtue of direct interactions with cells and bioactive degradation products . Most importantly, our in vivo studies indicated that GF/NS significantly improved BMP2-induced bone regeneration while it didn’t induce ectopic bone formation without BMP2 presence (Fig.1).
Conclusions: Therefore, we developed an innovative, facile, effective and safe strategy to improve the osteoblastic activity of the polymeric 3D scaffold by using the biocompatible, biodegradable and low lost cost synthetic 2D nanomaterial.
Methods: By using thermally induced phase separation (TIPS) method together with particle leaching technique (TIPS&P), a synthetic 2D nanosilicate (NS)-functionalized 3D gelatin nanofirous scaffold (GF) was developed to morphologically and chemically mimic native bone-matrix. The morphology and microstructure of 2D and 3D GF scaffolds were studied by using Quanta standard Environmental SEM (FEI, USA). The osteogenic differentiation of hMSCs on scaffolds were studied by testing the ALP activity, calcium content, and osteogenic gene expression. In addition to in vitro study, the effects of composite scaffolds on rhBMP2-induced bone formation were investigated by using an ectopic bone formation model in C57BL/6NHsd male mice (n=6).
Results: In addition to the significantly higher mechanical strength, the composite scaffolds (GF/NS) demonstrated significantly stronger ability to promote the osteogenic differentiation of hMSCs in vitro compared to the neat scaffold (GF). Our data further revealed that this intriguing pro-osteoblastic functionality was largely because of the unique features of the biodegradable nanoclay, particularly, the strong binding ability to pro-osteoblastic factors (e.g., BMP2 and small molecules) to achieve sustained release in addition to the intrinsic osteoinductivity by virtue of direct interactions with cells and bioactive degradation products . Most importantly, our in vivo studies indicated that GF/NS significantly improved BMP2-induced bone regeneration while it didn’t induce ectopic bone formation without BMP2 presence (Fig.1).
Conclusions: Therefore, we developed an innovative, facile, effective and safe strategy to improve the osteoblastic activity of the polymeric 3D scaffold by using the biocompatible, biodegradable and low lost cost synthetic 2D nanomaterial.
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