IADR Abstract Archives
Evaluation of Penetration Patterns of Fluorescein Related to Caries Activity
Objectives: To determine whether caries lesion will be progressed or not is essential for making treatment procedure. The Nyvad criteria, one of the visual methods for differentiating caries activity, is determined as trained clinician’s subjective impression. The fluorescence examination using the fluorescein sodium, which is able to go through micro-pore, might be an objective method for quantitative and qualitative differentiating caries activity. Therefore, this study aimed to objectively differentiate caries activity using the penetration of the fluorescein sodium.
Methods: 18 extracted human teeth were prepared. Caries activity of the teeth was evaluated as the Nyvad criteria by three examiners. Fluorescence images of the teeth were captured using the light induced-fluorescence technology-based device (QLF-D, Inspektor Research System BV, Amsterdam, the Netherlands) before and after dipping in 100μM fluorescein solution. The difference between the fluorescence images before and after made the green image using the image analysis software (Image-Pro Plus 6.0, Media Cybernetics, Silver Spring, USA). The green value, which indicates the amount of fluorescence from penetrated fluorescein was calculated in the green image. The difference of the green value was statistically evaluated by independent t-test.
Results: Teeth were classified as 11 active caries lesion and seven inactive caries lesion. The green images of active caries lesion showed distinctly green fluorescence of the penetrated fluorescein. On the contrary, those of inactive caries lesion showed rarely green fluorescence. The average (standard deviation) of the green value in active and inactive was 5.91 (8.79) and 0.46 (0.75), respectively. The green values between active and inactive caries lesion were statistically significant differences (P<0.01).
Conclusions: Caries activity would objectively differentiate with the penetration of the fluorescein sodium.
Methods: 18 extracted human teeth were prepared. Caries activity of the teeth was evaluated as the Nyvad criteria by three examiners. Fluorescence images of the teeth were captured using the light induced-fluorescence technology-based device (QLF-D, Inspektor Research System BV, Amsterdam, the Netherlands) before and after dipping in 100μM fluorescein solution. The difference between the fluorescence images before and after made the green image using the image analysis software (Image-Pro Plus 6.0, Media Cybernetics, Silver Spring, USA). The green value, which indicates the amount of fluorescence from penetrated fluorescein was calculated in the green image. The difference of the green value was statistically evaluated by independent t-test.
Results: Teeth were classified as 11 active caries lesion and seven inactive caries lesion. The green images of active caries lesion showed distinctly green fluorescence of the penetrated fluorescein. On the contrary, those of inactive caries lesion showed rarely green fluorescence. The average (standard deviation) of the green value in active and inactive was 5.91 (8.79) and 0.46 (0.75), respectively. The green values between active and inactive caries lesion were statistically significant differences (P<0.01).
Conclusions: Caries activity would objectively differentiate with the penetration of the fluorescein sodium.