IADR Abstract Archives
Selenomonas Noxia Location in the Oral Cavity of Pediatric Patients
Objectives: Selenomonas noxia (SN) is a gram-negative, obligate anaerobe that is unable to metabolize sucrose (unlike other Selenomonas species). It is found among healthy oral flora, but exhibits increased concentrations and prevalence in disease states, including generalized aggressive and chronic periodontitis, as well as gastric ulcers. Increasing numbers of studies have demonstrated an inter-relationship between SN and obesity, with greater numbers of overweight individuals exhibiting increased abundance of oral SN. The objective of this study was to evaluate the prevalence and location of SN among pediatric patients.
Methods: Using an IRB-approved protocol, samples were provided by pediatric patients from several oral locations, including lower incisor (lingual), dorsal tongue, upper molar (buccal), gingival crevicular fluid (maxillary central) using sterile paper points, as well as unstimulated saliva. DNA was subsequently isolated for qPCR screening and analysis.
Results: Forty seven (n=47) patients were screened, which ranged in age from five to fifteen years of age (average 10.3). Nearly two-thirds of patients were male (64.6%) with the vast majority identified as Hispanic (72.9%) or other minority (25.0%). DNA was successfully isolated from each of the 235 sampled sites with an overall average DNA concentration of 1.43 ug/uL and overall purity (A260:A80 ratio) of 1.88. Differential results have been observed by patient age, body mass index (BMI) and sampling site - with analysis on-going.
Conclusions: Although few studies have evaluated SN prevalence among pediatric patients, this study may be the first to provide site-specific oral location information, as well as data regarding age, race or ethnicity and BMI. It is hoped that these data may provide important information regarding the oral prevalence and location of SN among pediatric patients, which may help to improve our understanding of screening tools and potential health risks associated with this organism.
Methods: Using an IRB-approved protocol, samples were provided by pediatric patients from several oral locations, including lower incisor (lingual), dorsal tongue, upper molar (buccal), gingival crevicular fluid (maxillary central) using sterile paper points, as well as unstimulated saliva. DNA was subsequently isolated for qPCR screening and analysis.
Results: Forty seven (n=47) patients were screened, which ranged in age from five to fifteen years of age (average 10.3). Nearly two-thirds of patients were male (64.6%) with the vast majority identified as Hispanic (72.9%) or other minority (25.0%). DNA was successfully isolated from each of the 235 sampled sites with an overall average DNA concentration of 1.43 ug/uL and overall purity (A260:A80 ratio) of 1.88. Differential results have been observed by patient age, body mass index (BMI) and sampling site - with analysis on-going.
Conclusions: Although few studies have evaluated SN prevalence among pediatric patients, this study may be the first to provide site-specific oral location information, as well as data regarding age, race or ethnicity and BMI. It is hoped that these data may provide important information regarding the oral prevalence and location of SN among pediatric patients, which may help to improve our understanding of screening tools and potential health risks associated with this organism.