IADR Abstract Archives
Evaluations of Novel Hydrolytic Resistant Antibacterial Monomers for Resin Adhesive
Objectives: To evaluate the antibacterial properties of experimental quaternary ammonium methacrylamide and methacrylate monomers and to evaluate degree of conversion (DC%), microhardness, flexural strength and modulus of co-monomers made from co-polymerized each experimental monomer with relatively hydrophobic Resin2 (BisGMA/TEGDMA/CQ/EDMAB) and hydrophilic Resin3 (BisGMA/HEMA/CQ/EDMAB).
Methods: Four experimental monomers were synthesized at Faculty of Science, PSU. Two of them were methacrylamide monomers with 11 and 12 carbon alkyl chain named MAUPB and MADPB, respectively. The corresponding methacrylate monomers were also synthesized, MUPB and MDPB (control). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by serial microdilution assays using S. mutans and E. faecalis (n=3). Each monomer was incorporated into experimental Resin2 or Resin3 at 10wt%. DC% was determined by FTIR (n=5). Vickers microhardness test was performed (n=8). Same specimens were used for water sorption (WS) and solubility (WSL) measurements at 28-day and 3-month. Flexural strength and modulus were performed according to ISO 4049 at immediate and after 3-month storage (n=10). Friedman’s test or Kruskal Wallis was performed to determine significant differences of the variables. Mann-Whitney or Wilcoxon Signed Rank test was used to compare the difference between groups at p-value of 0.05 (SPSS 23.0).
Results: Antibacterial activity of MDPB≥MUPB>MADPB>MAUPB. The concentrations of these monomers in experimental resins at 10wt% were above their MBC. MADPB was not further evaluated due to high cost. Incorporation of MAUPB in Resin2 had no significant effect on DC% and immediate microhardness, while significantly increased DC% in Resin3. After 3-month storage, MAUPB contained resins illustrated significantly higher microhardness, higher flexural strength, and less WS than their corresponding resins containing MUPB. WSL decreased significantly by incorporation of either MUPB or MAUPB in both resins.
Conclusions: A quaternary ammonium methacrylamide monomer, MAUPB, is potentially used to formulate antibacterial hydrolytic resistant resin adhesives.
Methods: Four experimental monomers were synthesized at Faculty of Science, PSU. Two of them were methacrylamide monomers with 11 and 12 carbon alkyl chain named MAUPB and MADPB, respectively. The corresponding methacrylate monomers were also synthesized, MUPB and MDPB (control). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by serial microdilution assays using S. mutans and E. faecalis (n=3). Each monomer was incorporated into experimental Resin2 or Resin3 at 10wt%. DC% was determined by FTIR (n=5). Vickers microhardness test was performed (n=8). Same specimens were used for water sorption (WS) and solubility (WSL) measurements at 28-day and 3-month. Flexural strength and modulus were performed according to ISO 4049 at immediate and after 3-month storage (n=10). Friedman’s test or Kruskal Wallis was performed to determine significant differences of the variables. Mann-Whitney or Wilcoxon Signed Rank test was used to compare the difference between groups at p-value of 0.05 (SPSS 23.0).
Results: Antibacterial activity of MDPB≥MUPB>MADPB>MAUPB. The concentrations of these monomers in experimental resins at 10wt% were above their MBC. MADPB was not further evaluated due to high cost. Incorporation of MAUPB in Resin2 had no significant effect on DC% and immediate microhardness, while significantly increased DC% in Resin3. After 3-month storage, MAUPB contained resins illustrated significantly higher microhardness, higher flexural strength, and less WS than their corresponding resins containing MUPB. WSL decreased significantly by incorporation of either MUPB or MAUPB in both resins.
Conclusions: A quaternary ammonium methacrylamide monomer, MAUPB, is potentially used to formulate antibacterial hydrolytic resistant resin adhesives.
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