IADR Abstract Archives
Microbial Community in Extraradicular and Intraradicular Infections Associated with Persistent Apical Periodontitis
Objectives: Persistent inflammation have been found to be associated with bacterial biofilms surviving in the apical root canal system and/or on the root surface, and/or in the periapical lesions.The aim of this study is tocharacterize the bacterial community present in the extraradicular biofilm, periradicular lesions and matched root canal biofilm of root ends associated with persisitent apical periodontitis.
Methods: Eight adult patients who presented for apicoectomy of persistent periradicular lesions after endodontic therapy were selected for the study and total 29 samples of extraradicular biofilm, periradicular lesion and matched root canal biofilm of root ends were collected. The microbial communities were analyzed by high-through Illumina Hiseq sequencing.
Results: Bacteria were detected in all root canal, extraradicular biofilm and apical lesion samples. Twenty two phyla were detected and Bacteroidetes, Firmicutes, Proteobacteria,Actinobacteria were prominent.The prevalent bacterial genera in extraradicular and intraradicular infection were Streptococcus, Enterococcus, Bifidobacterium, Staphylococcus. The profile of bacterial community colonizing the apical third of infected root canals is as similar as that occurring at extraradicular regions. A high variability was observed for interindividual (samples from the same region but from different patients) comparison.
Conclusions: Persistent extraradicular and intraradicular infections are polymicrobial infections with many bacterial species. The similar bacterial profiles of both extraradicular and intraradicuar infections may be indicate that the concept of extraradicular infection being dependent on the intraradicular infection is true.
Methods: Eight adult patients who presented for apicoectomy of persistent periradicular lesions after endodontic therapy were selected for the study and total 29 samples of extraradicular biofilm, periradicular lesion and matched root canal biofilm of root ends were collected. The microbial communities were analyzed by high-through Illumina Hiseq sequencing.
Results: Bacteria were detected in all root canal, extraradicular biofilm and apical lesion samples. Twenty two phyla were detected and Bacteroidetes, Firmicutes, Proteobacteria,Actinobacteria were prominent.The prevalent bacterial genera in extraradicular and intraradicular infection were Streptococcus, Enterococcus, Bifidobacterium, Staphylococcus. The profile of bacterial community colonizing the apical third of infected root canals is as similar as that occurring at extraradicular regions. A high variability was observed for interindividual (samples from the same region but from different patients) comparison.
Conclusions: Persistent extraradicular and intraradicular infections are polymicrobial infections with many bacterial species. The similar bacterial profiles of both extraradicular and intraradicuar infections may be indicate that the concept of extraradicular infection being dependent on the intraradicular infection is true.