IADR Abstract Archives
Light-Induced Anisotropic Cell Sheet Technology for The Construction of Tissue-Engineered Periosteum
Objectives: The periosteum consists of an outer fibrous layer and a highly cellularised inner cambium layer, which plays a critical role in bone homeostasis and regeneration. Light-induced anisotropic cell sheet (LIASC) combines micropatterning approach and cell sheet technology, and has been applied in micro-structured thin-layer tissue replacement and cell-dense 3D tissue construction. In this study, it was used to mimic the structure and function of periosteum.
Methods: The anisotropic human mesenchymal stem cell sheets (A-hMSCs) were harvested by LIASC. The multilineage differentiation capacity of A-hMSCs was evaluated in vitro and then the cell sheets were co-cultured with endothelial cells (ECs) and pre-osteoblasts. In vivo study, the A-hMSCs were applied in the treatment of critical-size calvarial defect of nude mouse. Radiographic, histologic and histo-morphometric analyses were performed to evaluate the new bone formation inside the defects.
Results: It demonstrated the A-hMSCs had good viability and multilineage differentiation capacity after light-induced cell detachment. The arrangement and direction of cells and extracellular matrix in anisotropic cell sheets were similar with natural periosteum tissue. The A-hMSCs showed evident mineralized nodules, higher alkaline phosphatase activities and expression of related osteogenic genes than non-anisotropic hMSC sheets. Moreover, the A-hMSCs could induce the formation of capillary-like network of ECs and the directional migration and differentiation of pre-osteoblasts. In vivo results, the defects treated with A-hMSCs showed significantly more bone formation than non-anisotropic cell sheet group and empty group.
Conclusions: It indicates that the anisotropic hMSCs sheets have similar structure and function with natural periosteum, and enhances bone repair both in vitro and vivo, and it might be considered as potential substitutes for periosteum tissue.
Methods: The anisotropic human mesenchymal stem cell sheets (A-hMSCs) were harvested by LIASC. The multilineage differentiation capacity of A-hMSCs was evaluated in vitro and then the cell sheets were co-cultured with endothelial cells (ECs) and pre-osteoblasts. In vivo study, the A-hMSCs were applied in the treatment of critical-size calvarial defect of nude mouse. Radiographic, histologic and histo-morphometric analyses were performed to evaluate the new bone formation inside the defects.
Results: It demonstrated the A-hMSCs had good viability and multilineage differentiation capacity after light-induced cell detachment. The arrangement and direction of cells and extracellular matrix in anisotropic cell sheets were similar with natural periosteum tissue. The A-hMSCs showed evident mineralized nodules, higher alkaline phosphatase activities and expression of related osteogenic genes than non-anisotropic hMSC sheets. Moreover, the A-hMSCs could induce the formation of capillary-like network of ECs and the directional migration and differentiation of pre-osteoblasts. In vivo results, the defects treated with A-hMSCs showed significantly more bone formation than non-anisotropic cell sheet group and empty group.
Conclusions: It indicates that the anisotropic hMSCs sheets have similar structure and function with natural periosteum, and enhances bone repair both in vitro and vivo, and it might be considered as potential substitutes for periosteum tissue.