IADR Abstract Archives
The PCL/PVA/Adenosine Composite Nanofibers Promote Bone Regeneration
Objectives: This study aimed to facilitate bone regeneration by incorporating adenosine into the core of the poly (ε-caprolactone) (PCL) / polyvinyl alcohol (PVA) composite nanofibers.
Methods: The effect of adenosine on the osteogenic ability of rat bone mesenchymal stem cells (rBMSCs) was evaluated by alizarin red staining, immunohistochemistry staining, real-time PCR and western blot. The characterizations of the PCL/PVA composite membranes were evaluated by SEM, XPS, FT-IR, tensile tester, drop shape analysis system and high performance liquid chromatography. Then, we implanted the PCL/PVA composite membranes into the cranial defects and analyzed bone regeneration by Micro-CT, H&E, Masson's trichrome and immunohistochemical staining.
Results: We confirmed that adenosine (60μg/ml) could drive the BMSCs into mature osteoblasts, meanwhile stimulated the phosphorylation of STAT3 in BMSCs, which both could be dramatically inhibited by S3I-201 (specific STAT3 inhibitor). The PCL/PVA/Adenosine nanofibers with the 0.3 to 0.4 ratio of PVA and Adenosine exhibited good biocompatibilities and a uniform morphology and kept a sustained release of adenosine during a 30 days period. Compared to bare PCL/PVA scaffold, the PCL/PVA/Adenosine composite membrane displayed a higher compressive strength. After implanted in the cranial defect site, PCL/PVA/Adenosine membranes showed more robust promotion in bone regeneration when compared with those of the bare PCL/PVA groups both at 4 weeks and 8 weeks. However, this promotion effect could be suppressed by intraperitoneal injection of S3I-201 (1mg/kg) every two days.
Conclusions: These findings represent that adenosine promote the osteogenic ability of BMSCs by activating the STAT3 signaling pathway. Furthermore, the PCL/PVA coaxial electrospinning membrane could be a promising drug sustained-release system to release adenosine for the restoration of large bone defects.
Methods: The effect of adenosine on the osteogenic ability of rat bone mesenchymal stem cells (rBMSCs) was evaluated by alizarin red staining, immunohistochemistry staining, real-time PCR and western blot. The characterizations of the PCL/PVA composite membranes were evaluated by SEM, XPS, FT-IR, tensile tester, drop shape analysis system and high performance liquid chromatography. Then, we implanted the PCL/PVA composite membranes into the cranial defects and analyzed bone regeneration by Micro-CT, H&E, Masson's trichrome and immunohistochemical staining.
Results: We confirmed that adenosine (60μg/ml) could drive the BMSCs into mature osteoblasts, meanwhile stimulated the phosphorylation of STAT3 in BMSCs, which both could be dramatically inhibited by S3I-201 (specific STAT3 inhibitor). The PCL/PVA/Adenosine nanofibers with the 0.3 to 0.4 ratio of PVA and Adenosine exhibited good biocompatibilities and a uniform morphology and kept a sustained release of adenosine during a 30 days period. Compared to bare PCL/PVA scaffold, the PCL/PVA/Adenosine composite membrane displayed a higher compressive strength. After implanted in the cranial defect site, PCL/PVA/Adenosine membranes showed more robust promotion in bone regeneration when compared with those of the bare PCL/PVA groups both at 4 weeks and 8 weeks. However, this promotion effect could be suppressed by intraperitoneal injection of S3I-201 (1mg/kg) every two days.
Conclusions: These findings represent that adenosine promote the osteogenic ability of BMSCs by activating the STAT3 signaling pathway. Furthermore, the PCL/PVA coaxial electrospinning membrane could be a promising drug sustained-release system to release adenosine for the restoration of large bone defects.