IADR Abstract Archives
Bone Graft Evaluation of Patient with Cleft Palate - A Simulation in Mice
Objectives:
Clinically, the success rate of bone graft in adults with cleft palate is very low due to the bone reabsorption. The aim of this study is to simulate in mice the bone grafts that are made in patients with cleft palate to analyze the density and time of new bone formation by comparing 3D micro-computed tomography images.
Methods: Bone defects were created on the palate of the mice using a diamond spherical drill number 8, in order to simulate the maxillary defect present in patients with cleft palate.
Twenty five male mice were used, C57 / BL6 (n = 5 per group) to be divided in five experimental groups (Table 1).
The femur was removed from one donor animal, macerated (formation of the "ground bone") and used as a bone graft.
The "ground bone" was placed on the right side of the experimental animals, thus simulating the cleft palate; the left side of the maxilla was used as control. The maxilla collected after the euthanasia was used to assess the trabecular and cortical bone morphology using high-resolution micro-computed tomography (mCT).
Results: The results demonstrated that the autogenous bone graft assisted the reconstruction of the neoformed bone in the maxillary defect of the mouse. It was observed that after 24 hours the bone graft side presented higher bone mineral density (BMD, g / cm3), percentage of bone volume (BV / TV%) and bone volume (BV, mm3). After 7 and 14 days, there was no significant difference for the non-grafted side. On the grafted side there was a bone resorption that remained until the 14th day. The analyzes performed 21 days after surgery showed a significant bone neoformation on the side where the bone graft was done, and reaching the expected levels of neoformed bone (Figure 2). On the side where the autogenous graft was not placed, there was no significant neoformation and did not reach the adequate bone formation level.
Conclusions: The autogenous bone graft is very important for a new bone formation, showing success in the bone regeneration comparing the side of the maxilla with bone graft to the control side without bone graft.
Clinically, the success rate of bone graft in adults with cleft palate is very low due to the bone reabsorption. The aim of this study is to simulate in mice the bone grafts that are made in patients with cleft palate to analyze the density and time of new bone formation by comparing 3D micro-computed tomography images.
Methods: Bone defects were created on the palate of the mice using a diamond spherical drill number 8, in order to simulate the maxillary defect present in patients with cleft palate.
Twenty five male mice were used, C57 / BL6 (n = 5 per group) to be divided in five experimental groups (Table 1).
The femur was removed from one donor animal, macerated (formation of the "ground bone") and used as a bone graft.
The "ground bone" was placed on the right side of the experimental animals, thus simulating the cleft palate; the left side of the maxilla was used as control. The maxilla collected after the euthanasia was used to assess the trabecular and cortical bone morphology using high-resolution micro-computed tomography (mCT).
Results: The results demonstrated that the autogenous bone graft assisted the reconstruction of the neoformed bone in the maxillary defect of the mouse. It was observed that after 24 hours the bone graft side presented higher bone mineral density (BMD, g / cm3), percentage of bone volume (BV / TV%) and bone volume (BV, mm3). After 7 and 14 days, there was no significant difference for the non-grafted side. On the grafted side there was a bone resorption that remained until the 14th day. The analyzes performed 21 days after surgery showed a significant bone neoformation on the side where the bone graft was done, and reaching the expected levels of neoformed bone (Figure 2). On the side where the autogenous graft was not placed, there was no significant neoformation and did not reach the adequate bone formation level.
Conclusions: The autogenous bone graft is very important for a new bone formation, showing success in the bone regeneration comparing the side of the maxilla with bone graft to the control side without bone graft.
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