IADR Abstract Archives
Can Silver Diammine Fluoride and Glass Ionomer Remineralize Natural Dentin Caries?
Objectives: Silver diammine fluoride (SDF) is an increasingly popular anti-cariogenic agent for minimally-invasive dentistry. This study addressed a common question regarding an optimal application frequency of SDF when used with glass ionomer cement (GI) for remineralizing dentin in advanced carious lesions.
Methods: 25 freshly extracted human posterior permanent teeth with advanced carious lesions were used in this study. After superficial removal of infected dentin, single (G3), double (G4), and triple (G5) applications of 38% SDF (Advantage Arrest) followed by a layer of GI (GC Fuji IX GP) were compared to no treatment (negative control, G2), and GI only (G1). All teeth were stored in artificial saliva (AS) solution for 2 weeks after final treatment. Micro-computed X-ray tomography (mCT) scans were obtained at each stage and analyzed using Amira (FEI VG) and ImageJ (NIH) to plot mineral density vs. depth and calculate lesion depth (LD) and mineral loss (DZ) for 30 lesions (n=30) per treatment group. Comparisons within and between groups with appropriate parametric and non-parametric statistical tests were carried out (α=0.05) using SPSS software (IBM).
Results: Mean LD and DZ at baseline ranged from 638 to 1008 µm and 2327 to 10013 µm.vol%. Paired t-test showed that LD and DZ changed significantly within all groups from baseline to treatment weeks following AS storage (p<0.05) except for LD in the control (p>0.05). Mean reductions in groups G1 to G5 were 6.8%, 0.4%, 19.5%, 13.2% and 10.6% for LD, and 49.1%, 11.3%, 54.3%, 66.1% and 48.1% for DZ, respectively. One-way ANOVA showed significant difference among groups for both variables (p<0.05). Collectively, G3 and G4 showed the highest mineral gains.
Conclusions: The remineralization effect of SDF when used in conjunction with AS solution and GI is enhanced. One or two applications of SDF are effective in healing advanced dentin lesions with additional application not demonstrating additional benefit.
Methods: 25 freshly extracted human posterior permanent teeth with advanced carious lesions were used in this study. After superficial removal of infected dentin, single (G3), double (G4), and triple (G5) applications of 38% SDF (Advantage Arrest) followed by a layer of GI (GC Fuji IX GP) were compared to no treatment (negative control, G2), and GI only (G1). All teeth were stored in artificial saliva (AS) solution for 2 weeks after final treatment. Micro-computed X-ray tomography (mCT) scans were obtained at each stage and analyzed using Amira (FEI VG) and ImageJ (NIH) to plot mineral density vs. depth and calculate lesion depth (LD) and mineral loss (DZ) for 30 lesions (n=30) per treatment group. Comparisons within and between groups with appropriate parametric and non-parametric statistical tests were carried out (α=0.05) using SPSS software (IBM).
Results: Mean LD and DZ at baseline ranged from 638 to 1008 µm and 2327 to 10013 µm.vol%. Paired t-test showed that LD and DZ changed significantly within all groups from baseline to treatment weeks following AS storage (p<0.05) except for LD in the control (p>0.05). Mean reductions in groups G1 to G5 were 6.8%, 0.4%, 19.5%, 13.2% and 10.6% for LD, and 49.1%, 11.3%, 54.3%, 66.1% and 48.1% for DZ, respectively. One-way ANOVA showed significant difference among groups for both variables (p<0.05). Collectively, G3 and G4 showed the highest mineral gains.
Conclusions: The remineralization effect of SDF when used in conjunction with AS solution and GI is enhanced. One or two applications of SDF are effective in healing advanced dentin lesions with additional application not demonstrating additional benefit.