IADR Abstract Archives
Caffeic Acid Phenethyl Ester Effects On Dental Pulp Stem Cells
Objectives: Caffeic acid phenethyl ester or CAPE is an organic molecule produced by honeybees, which has been previously evaluated for the anti-inflammatory effects on mast cells, as well as for the anti-tumor effects in many types of cancer – including oral cancers. However, no studies to date have evaluated any potential positive effects of CAPE on dental pulp stem cells (DPSC). Based upon the lack of information regarding the effects of CAPE, this study sought to evaluate the effects of CAPE on cellular viability and proliferation in three, primary DPSC explants.
Methods: Using three primary DPSC explants (dpsc-3882, dpsc-5423, dpsc-8604) CAPE was administered at previously identified bioactive concentrations (20 uM, 100 uM, 200 uM) in three day in vitro assays. Trypan blue exclusion assays were used to determine any effects on cellular viability and proliferation was measured using 96-well microplate assays.
Results: The results from this study demonstrated CAPE induced differential effects on each of the DPSC isolates. For example, CAPE induced an increase in proliferation of dpsc-3882 cells between 9.8-23.4% over the range [100-200 uM] (viability up to 42.5%). Similarly, dpsc-8604 growth increased up to 64% (viability up to 67%), while dpsc-5423 growth increased up to 79.9% over the same concentration range (viability up to 74%).
Conclusions: Although these data are from a preliminary study of the effects of CAPE on DPSC, the nearly uniform effects on cellular proliferation over the biologically relevant range of [100-200 uM] provides evidence of the positive effects of this compound on cellular growth.
Methods: Using three primary DPSC explants (dpsc-3882, dpsc-5423, dpsc-8604) CAPE was administered at previously identified bioactive concentrations (20 uM, 100 uM, 200 uM) in three day in vitro assays. Trypan blue exclusion assays were used to determine any effects on cellular viability and proliferation was measured using 96-well microplate assays.
Results: The results from this study demonstrated CAPE induced differential effects on each of the DPSC isolates. For example, CAPE induced an increase in proliferation of dpsc-3882 cells between 9.8-23.4% over the range [100-200 uM] (viability up to 42.5%). Similarly, dpsc-8604 growth increased up to 64% (viability up to 67%), while dpsc-5423 growth increased up to 79.9% over the same concentration range (viability up to 74%).
Conclusions: Although these data are from a preliminary study of the effects of CAPE on DPSC, the nearly uniform effects on cellular proliferation over the biologically relevant range of [100-200 uM] provides evidence of the positive effects of this compound on cellular growth.