Novel Dentifrice Containing Stabilized Chlorine Dioxide, Sarkosyl, and Sodium Fluoride

Objectives: This study evaluates effectiveness of a novel dentifrice containing stabilized chlorine dioxide, sodium lauroyl sarcosinate, and sodium fluoride for plaque removal, enamel fluoride uptake, enamel remineralization and inhibition of biofilm regrowth.
Methods: Assessment of pellicle cleaning and plaque removal utilized a standard 800 strokes of 4.5 minutes to remove artificially induced staining by different dentifrices and scored based on the pellicle cleaning ratio (PCR). Fluoride content of each enamel specimen were determined using a micro-drill technique to a depth of 100 µm and 20-day Surface Micro Hardness (SMH) assessments were conducted. For determining the efficacy of the dentifrices’ inhibition of polymicrobial biofilm regrowth, artificial biofilm was established onto teeth specimen then brushed for 30 seconds. The specimens were rinsed with sterile water and the remaining oral biofilm on the enamel sections was incubated for 24 hours, followed by counting of the colonies on the agar plates.
Results: The mean pellicle cleaning ratio of CloSYS® Toothpaste B, CloSYS® Toothpaste C, and ADA Reference Material were 95.9, 101.21, and 100.0, respectively. The fluoride uptake by Toothpaste C, current commercial product, was 96.1%, 107.7%, and 303.3% higher than USP Reference Dentifrice, Colgate Total Advanced Whitening, and Crest 3 D White Mild Mint toothpastes, respectively. The remineralization after 20 days by Toothpaste C was 37.6%, 80.9%, and 103.0% higher than USP Reference Dentifrice, Colgate Total Advanced Whitening, and Crest 3 D White Mild Mint toothpastes, respectively. There was a significant reduction in regrowth of oral polymicrobial biofilm obtained by Toothpaste C compared to the water brushed control (p<0.05).
Conclusions: There is synergism exhibited between sodium fluoride, stabilized chlorine dioxide, and sodium lauroyl sarcosinate as indicated by the superior efficacy of the novel dentifrice compared to other commercial toothpastes and controls that were tested.