Neovestitol-vestitol Fraction (from Brazilian Red Propolis) Reduces Subgingival Multispecies Biofilm

Objectives: The aim of the present work is to evaluate the NV effects on subgingival multispecies biofilm in vitro model.
Methods: The subgingival biofilm containing 32 species related to periodontitis was formed for 7 days using the Calgary device. Two treatments of 1 min each per day were performed since day 3 using neovestitol-vestitol (NV) fraction at 1600 and 800 µg/mL. Vehicle-control (negative) and chlorhexidine 0.12% (positive) were used as controls. After 7 days of biofilm formation, the metabolic activity and the microbial composition were evaluated by colorimetric (TTC reaction) and DNA-DNA hybridization, respectively. All results were statistically analyzed (ANOVA, Tukey’s, p<0.05).
Results: NV at 800 and 1600 μg/mL significantly reduced biofilm metabolic activity by 48% and 68%, respectively, when compared to vehicle-control. When compared to chlorhexidine results (reduction of 60 %), there was no statistical significance among the tested groups. NV 1600 and 800 and chlorhexidine reduced counting of 18, 10 and 17 species, respectively. NV 1600 and chlorhexidine were statistically similar effective against target microorganisms of periodontal disease etiology such as P. gingivalis, F. nucleatum and F. periodonticum.
Conclusions: NV has been shown to be a promising antimicrobial agent that may be useful in the control of periodontal disease. However, new studies using in vivo models of experimental periodontal disease should be performed to corroborate with such effect.