IADR Abstract Archives
Polyphenolic Concentration and Effect of Peruvian Propolis on Streptococcus mutans
Objectives: Compare the Polyphenolic concentration of six Peruvian propolis and their antibacterial effect against strains of S. mutans.
Correlate the concentration of polyphenols with the antibacterial effect of six Peruvian propolis on strains of S. mutans.
Methods: Samples were taken from six regions of Peru: Arequipa, Cusco, Huaraz, Huánuco, Huamachuco and Oxapampa, all were masked by a person outside the study with the letters A, B, C, D, E and F; and later taken to the Pharmacy and Biochemistry Laboratory of the Los Angeles Catholic University of Chimbote to make the extracts in different concentrations obtained from the maximum solubility. Subsequently, the biological tests were carried out in the Bacteriology laboratory of the Universidad Peruana Cayateno Heredia where the antimicrobial effect on strains of S. mutans ATCC 25175 was evaluated by means of the wells technique to measure the inhibition halo. Likewise, the concentration of total polyphenols was carried out for all the extracts.
Results: The results of this study show that the extract with the highest antimicrobial effect is that collected in Cusco at 10% with a halo sizes of 19 mm, followed by the extract obtained from Arequipa at 10% with diameter sizes of 18.5 mm followed by the 5% with a halo of 16.5 mm, 2.5% with a halo sizes of 14 mm, followed by the extract of Huamachuco at 2% with a halo sizes of 12 mm, followed by 1% of 11 mm and 0.5% also of 11 mm; the extract of Oxapampa at 2% with 10.5 mm, finally the extracts that showed less effect were Huánuco and Huaraz that at 2% and 5% respectively managed to form a halo sizes of 10 mm. These results reflect that the highest concentrations influence the antimicrobial effect against strains of S. mutans. Finally, in the evaluation of the correlation of polyphenols and antibacterial effect, it was estimated at 0.757, which implies a direct relationship between both variables, with a significant difference; This is because polyphenols are the main source of antibacterial action.
Conclusions: The six Peruvian propolis evaluated showed an inhibitory effect on the growth of strains of S. mutans, discarding by their antibacterial effect those from the regions of Cusco and Arequipa. There is a relationship between the concentration of polyphenols and the antibacterial effect of the samples evaluated
Correlate the concentration of polyphenols with the antibacterial effect of six Peruvian propolis on strains of S. mutans.
Methods: Samples were taken from six regions of Peru: Arequipa, Cusco, Huaraz, Huánuco, Huamachuco and Oxapampa, all were masked by a person outside the study with the letters A, B, C, D, E and F; and later taken to the Pharmacy and Biochemistry Laboratory of the Los Angeles Catholic University of Chimbote to make the extracts in different concentrations obtained from the maximum solubility. Subsequently, the biological tests were carried out in the Bacteriology laboratory of the Universidad Peruana Cayateno Heredia where the antimicrobial effect on strains of S. mutans ATCC 25175 was evaluated by means of the wells technique to measure the inhibition halo. Likewise, the concentration of total polyphenols was carried out for all the extracts.
Results: The results of this study show that the extract with the highest antimicrobial effect is that collected in Cusco at 10% with a halo sizes of 19 mm, followed by the extract obtained from Arequipa at 10% with diameter sizes of 18.5 mm followed by the 5% with a halo of 16.5 mm, 2.5% with a halo sizes of 14 mm, followed by the extract of Huamachuco at 2% with a halo sizes of 12 mm, followed by 1% of 11 mm and 0.5% also of 11 mm; the extract of Oxapampa at 2% with 10.5 mm, finally the extracts that showed less effect were Huánuco and Huaraz that at 2% and 5% respectively managed to form a halo sizes of 10 mm. These results reflect that the highest concentrations influence the antimicrobial effect against strains of S. mutans. Finally, in the evaluation of the correlation of polyphenols and antibacterial effect, it was estimated at 0.757, which implies a direct relationship between both variables, with a significant difference; This is because polyphenols are the main source of antibacterial action.
Conclusions: The six Peruvian propolis evaluated showed an inhibitory effect on the growth of strains of S. mutans, discarding by their antibacterial effect those from the regions of Cusco and Arequipa. There is a relationship between the concentration of polyphenols and the antibacterial effect of the samples evaluated
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