IADR Abstract Archives
Neural crest-related Stem Cell Homing and Alveolar Bone Regeneration
Objectives: The amplification of autologous NCSCs potential in homing for self-repair/regeneration might be considered as an alternative therapy except for traditional cell transplantation. Here we broadly define cell homing as active recruitment of endogenous cells, including stem/progenitor cells, into an anatomic compartment. The purpose of this study was to evaluate the potential of Adult Palatinum as a Novel source of stem cells in combination with transplantation of allogen cortico-spongiosa Bone Rings for one-stage bone augmentation and immediate implant placement.
Methods: We used allogen bone rings and added subepithelial soft tissue taken from palate. Several recent studies showed that neural crest-related stem cells can be isolated from palate. These cells have in common that they form neurosphere-like clusters and proliferate in serum-free media. 15 subjects were included (ethics committee, Muenster, Germany, f-s, 17/02/2014) and were allocated either to the upper jaw subgroup or to the lower jaw subgroup. 3D DVT-analysis and qualitative histological analysis on non-decalcified sections using Technovit 9100 New with different stains were carried out.
Results: In both subgroups, the remaining particles of the bone substitute material were closely surrounded by newly formed bone. NCSCs were found to aggregate in the periimplant niches and emerge in newly-formed bones or fibers. A magnification of those areas revealed specific cells generally involved in bone regenerating processes (osteocytes, osteoblasts and osteoclasts), osteoid, vessels, and collagen connective tissue. These results indicated that NCSCs might contribute to the formation of new fibers and periimplant bone tissue during periimplant bone regeneration.
Conclusions: Induced homing of human neural crest-related stem cells (hNCSCs) from the human palate overcome some of the key scientific and regulatory issues associated with stem cell transplantation. In conclusion, autologous NCSCs were capable of homing into the surgical sites created by implant placement and participating in periimplant bone tissue repair.
Methods: We used allogen bone rings and added subepithelial soft tissue taken from palate. Several recent studies showed that neural crest-related stem cells can be isolated from palate. These cells have in common that they form neurosphere-like clusters and proliferate in serum-free media. 15 subjects were included (ethics committee, Muenster, Germany, f-s, 17/02/2014) and were allocated either to the upper jaw subgroup or to the lower jaw subgroup. 3D DVT-analysis and qualitative histological analysis on non-decalcified sections using Technovit 9100 New with different stains were carried out.
Results: In both subgroups, the remaining particles of the bone substitute material were closely surrounded by newly formed bone. NCSCs were found to aggregate in the periimplant niches and emerge in newly-formed bones or fibers. A magnification of those areas revealed specific cells generally involved in bone regenerating processes (osteocytes, osteoblasts and osteoclasts), osteoid, vessels, and collagen connective tissue. These results indicated that NCSCs might contribute to the formation of new fibers and periimplant bone tissue during periimplant bone regeneration.
Conclusions: Induced homing of human neural crest-related stem cells (hNCSCs) from the human palate overcome some of the key scientific and regulatory issues associated with stem cell transplantation. In conclusion, autologous NCSCs were capable of homing into the surgical sites created by implant placement and participating in periimplant bone tissue repair.