IADR Abstract Archives
Temu Kunci (Boesenbergia-rotunda) Extract Antibiofilm Activity Against Root-canal Pathogen Biofilms
Objectives: Bacteria, such as Enterococcus faecalis, Fusobacterium nucleatum,and Treponema denticola, are one of the factors associated with endodontic treatment failure. These bacteria can form biofilm and become more resistant to root canal irrigation. A medicinal extract from an Indonesian plant, temu kunci (Boesenbergia rotunda), is known to have an antibacterial effect. Objective of this study is to determine the antibiofilm effects of temu kunci extract against Enterococcus faecalis, Fusobacterium nucleatum and Treponema denticola.
Methods: This experimental laboratory study used the post-test control group design method. The E. faecalis, F. nucleatum, and T. denticola were each cultured in Brain Heart Infusion (BHI) broth, for 24 hours, at 37oC, under anaerobic conditions. Each bacteria was distributed into 96 microplate wells and treated with an ethanol extract of temu kunci in different concentrations and incubated for 5 minutes, 1h, 6h, and 24h. The biofilm without treatment was used as the negative control and sodium hypochlorite (NaOCl) was used as the positive control. The biofilm mass was read by using a microplate reader (490 nm). One-way analysis of variance (ANOVA) and a post-hoc test were used to analyze the results.
Results: The temu kunci extract significantly reduced E. faecalis,F. nucleatum, and T. denticola in the monospecies and multispecies biofilms for all incubation periods (p <0.05). The inhibitory efficiency of each concentration of the temu kunci extracts in preventing biofilm formation against E. faecalis, T. denticola, F. nucleatum monospecies and multispecies, respectively, is shown in Figure 1-4. After examining the biofilm formation, it was concluded that the type of bacteria, the concentration, and the incubation time period were statistically significant (p<0.05).
Conclusions: Temu kunci has antibiofilm activity against root canal pathogen biofilms, and it may be used as an alternative for root canal irrigation. However, further studies are needed to confirm this result.
Methods: This experimental laboratory study used the post-test control group design method. The E. faecalis, F. nucleatum, and T. denticola were each cultured in Brain Heart Infusion (BHI) broth, for 24 hours, at 37oC, under anaerobic conditions. Each bacteria was distributed into 96 microplate wells and treated with an ethanol extract of temu kunci in different concentrations and incubated for 5 minutes, 1h, 6h, and 24h. The biofilm without treatment was used as the negative control and sodium hypochlorite (NaOCl) was used as the positive control. The biofilm mass was read by using a microplate reader (490 nm). One-way analysis of variance (ANOVA) and a post-hoc test were used to analyze the results.
Results: The temu kunci extract significantly reduced E. faecalis,F. nucleatum, and T. denticola in the monospecies and multispecies biofilms for all incubation periods (p <0.05). The inhibitory efficiency of each concentration of the temu kunci extracts in preventing biofilm formation against E. faecalis, T. denticola, F. nucleatum monospecies and multispecies, respectively, is shown in Figure 1-4. After examining the biofilm formation, it was concluded that the type of bacteria, the concentration, and the incubation time period were statistically significant (p<0.05).
Conclusions: Temu kunci has antibiofilm activity against root canal pathogen biofilms, and it may be used as an alternative for root canal irrigation. However, further studies are needed to confirm this result.
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