IADR Abstract Archives
Combined Drug Delivery From Light Cured Nanocomposite Hydrogels
Objectives: Inadequate oral hygiene can lead serious consequences e.g. chronic oral inflammatory diseases like periodontitis. Combination of antiseptic, and antibiotic drugs can help us to reach maximum efficiency beside can minimalize the side effects. In this way the treatment could be shorter and the healing would be faster. The possibility of visible-light polymerization ensures higher flexibility of application as a local drug delivery system.
The aim of our study was the evaluation of the effectiveness of the biodegradable polymer based nanocomposite hydrogels as a locally useable combined drug delivery system.
Methods: The poly-γ-glutamic acid (PGA) polymer were modified by methacryloyl-groups to ensure the photopolymerizable properties of all components, and it was crosslinked previously for creation of nanoparticles (NP). This two part - the polymer matrix (MPGA), and the nanoparticles (MPGA-NP) - were loaded with chlorhexidine digluconate (CHX), and metronidazole (MET). The structure of the created nanocomposite hydrogels was analyzed by Scanning electron microscopy, and the release profiles of the drugs were studied by HPLC. The biocompatibility of the system was determined by Alamar-blue assay, and the antibiotic efficiency was presented by microbiological method.
Results: The NMR results and the mechanical stability of the nanocomposite hydrogels prove the successfulness of modifications, and photopolymerizable properties. The release kinetics of the different drugs showed, that beside a shorter (6hours), but more intensive MET emission can reach the system a lower, but prolonged (168hours) CHX level. Alamar-blue test evidenced the biocompability of the nanocomposite on osteosarcoma cell line, and showed antibiotic effect against E. coli.
Conclusions: This photopolymerizable local drug delivery system can be an alternative and effective method for local combination of antibiotic and antiseptic treatment which may guarantee the clinical effective concentration of the drugs with the using of minimal applicable dose.
The aim of our study was the evaluation of the effectiveness of the biodegradable polymer based nanocomposite hydrogels as a locally useable combined drug delivery system.
Methods: The poly-γ-glutamic acid (PGA) polymer were modified by methacryloyl-groups to ensure the photopolymerizable properties of all components, and it was crosslinked previously for creation of nanoparticles (NP). This two part - the polymer matrix (MPGA), and the nanoparticles (MPGA-NP) - were loaded with chlorhexidine digluconate (CHX), and metronidazole (MET). The structure of the created nanocomposite hydrogels was analyzed by Scanning electron microscopy, and the release profiles of the drugs were studied by HPLC. The biocompatibility of the system was determined by Alamar-blue assay, and the antibiotic efficiency was presented by microbiological method.
Results: The NMR results and the mechanical stability of the nanocomposite hydrogels prove the successfulness of modifications, and photopolymerizable properties. The release kinetics of the different drugs showed, that beside a shorter (6hours), but more intensive MET emission can reach the system a lower, but prolonged (168hours) CHX level. Alamar-blue test evidenced the biocompability of the nanocomposite on osteosarcoma cell line, and showed antibiotic effect against E. coli.
Conclusions: This photopolymerizable local drug delivery system can be an alternative and effective method for local combination of antibiotic and antiseptic treatment which may guarantee the clinical effective concentration of the drugs with the using of minimal applicable dose.