Cytocompatibility of Nano-porous Silica Using for Novel Controlled Drug-release Materials

Objectives: Nano-porous micro silica particles (NPMS) were known to have sustained molecules release property because of their large surface area and nanostructured cavities. This property can allow to fabricate widely fields. In this study, we investigated on cytocompatibility and drug-release property of NPMS.
Methods: NMPS (SIGMA-ALDRICH, St. Louis, MO) were characterized using SEM and TEM. They exposed to human alveolar epithelial cells (A549) and mouse osteoblastic cells (MC3T3-E1) which cultivated in D-MEM at 37°C in 5% CO₂. After the cultivation under NPMS exposure, their cell viability were determined NPMS contained glass ionomer cement (f10 x 1 mm) were prepared. After charge a model drug, the specimen were immersed in distilled water. The water exchanged every day for 2 weeks. For determine drug amount, obtained supernatant was analyzed using a UV-vis spectrometer.
Results: When A549 cells were exposed to NMPS with several concentration, they showed high viability even the concentration reached at 30 ppm. In addition, the viability under 10 ppm exposure remained as high as that of control even 3 days. In contrast, cells under CuO particle exposure shows the viability drastically decrease depending on the concentration. In the case of MC3T3-E1, they also similar tendency. These results suggest that NMPS have excellent cytocompatibility. In drug-release property test, NPMS contained specimen sustainably released model drug for 2 weeks. In contrast, cells under CuO exposure showed the viability decrease drastically depending on concentration.
Conclusions: NPMS indicated excellent drug-release property and cytocompatibility. These result suggested that it can be fabricate for a novel dental material with sustained drug-release property.