IADR Abstract Archives
Sesamol Induces Apoptosis in Human Oral Squamous Carcinoma Cells
Objectives: In this study we investigated the anticancer potential of sesamol against human oral squamous cell carcinoma (SCC-25) cells
Methods: Cytotoxic effect of Sesamol was determined by MTT assay, using different concentrations. Sesamol was treated SCC-25 cells with 62.5, 125 and 250 µM. Reactive oxygen species (ROS) generation was investigated by the dichlorofluorescein fluorescent staining assay. Dual acridine orange/ethidium bromide (AO/EB) fluorescent stained SCC-25 cells were visualized under a fluorescent microscope for apoptosis related membrane damage. Caspase 3 activity was done by double immunofluorescence assay. CDK1B gene and protein expression was evaluated by RT-PCR and western blotting analysis respectively.)
Results: Sesamol treatment caused significant cytotoxic effect in SCC-25 cells. Sesamol treatment also induced significant ROS generation and caspase activity in SCC-2 cells. Dual staining confirmsthe membrane and DNA damage pertaining to apoptosis. Sesamol significantly reduced the protein and gene level expression of CDK1B in OSCC cells.
Conclusions: Sesamol has the ability to induce ROS meditated apoptotic changes in OSCC cells and these results suggest that sesamol can be used as a modulating agent in oral squamous cell carcinoma.
Methods: Cytotoxic effect of Sesamol was determined by MTT assay, using different concentrations. Sesamol was treated SCC-25 cells with 62.5, 125 and 250 µM. Reactive oxygen species (ROS) generation was investigated by the dichlorofluorescein fluorescent staining assay. Dual acridine orange/ethidium bromide (AO/EB) fluorescent stained SCC-25 cells were visualized under a fluorescent microscope for apoptosis related membrane damage. Caspase 3 activity was done by double immunofluorescence assay. CDK1B gene and protein expression was evaluated by RT-PCR and western blotting analysis respectively.)
Results: Sesamol treatment caused significant cytotoxic effect in SCC-25 cells. Sesamol treatment also induced significant ROS generation and caspase activity in SCC-2 cells. Dual staining confirmsthe membrane and DNA damage pertaining to apoptosis. Sesamol significantly reduced the protein and gene level expression of CDK1B in OSCC cells.
Conclusions: Sesamol has the ability to induce ROS meditated apoptotic changes in OSCC cells and these results suggest that sesamol can be used as a modulating agent in oral squamous cell carcinoma.