Genome-wide Gene-expression Analysis in Mouse Hippocampus Stimulated With P. gingivalis LPS
Objectives: Periodontal diseases are one of the risk factors for systemic diseases such as diabetes and cardiovascular diseases. The involvement of periodontal pathogens in dementia has been recently suggested. The mechanism of the involvement has not been, however, shown thus far. In the present study, we examined a genome-wide analysis of the genes related to dementia in the mouse hippocampus stimulated with intraperitoneal administration of lipopolysaccharide (LPS) derived from P. gingivalis. Methods: Eight to ten week-old C57BL/6J mice and SAM-P/8 mice were administrated LPS derived from P. gingivalis intraperitoneally once every 3 days for a month. The normal serine was administrated as a negative control. We extracted the hippocampus from those mice after death and examined morphological analysis, DNA microarray, and quantitative RT-PCR (qRT-PCR). Results: No obvious inflammatory changes were observed in morphological analysis in either group. DNA microarray revealed that there were 317 genes with a more than 4-fold expression following stimulation with LPS, and that there were 388 genes with a less than 0.25-fold expression following it. We found a membrane metallo-endopeptidase (MME) gene related to Alzheimer’s dementia out of those genes. By qRT-PCR, a significant decreased expression of MME was confirmed (p<0.05). Conclusions: These results suggest LPS derived from P. gingivalis may be involved in AD with altered expression of genes related to AD.
Division: IADR/PER General Session
Meeting:2018 IADR/PER General Session (London, England) Location: London, England
Year: 2018 Final Presentation ID:3045 Abstract Category|Abstract Category(s):Microbiology/Immunology
Authors
Morikawa, Tetsuro
( Health Sciences University of Hokkaido
, Ishikari-Tobetu
, Hokkaido
, Japan
)
Paudel, Durga
( Health Sciences University of Hokkaido
, Ishikari
, Japan
)
Yoshida, Koki
( Health Sciences University of Hokkaido
, Ishikari-Tobetsu
, Hokkaido
, Japan
)
Sato, Jun
( Health Sciences Universityof Hokkaido
, Ishikari
, Japan
)
Matsuoka, Hirofumi
( Health Sciences University of Hokkaido
, Ishikari
, Japan
)
Nishimura, Michiko
( Health Sciences University of Hokkaido
, Ishikari-Tobetsu
, Hokkaido
, Japan
)
Saitoh, Masato
( Health Sciences University of Hokkaido
, Sapporo
, Japan
)
Hikita, Kazuhiro
( Health Sciences University of Hokkaido
, Ishikari-Tobetu
, Hokkaido
, Japan
)
Chiba, Itsuo
( Health Sciences University of Hokkaido
, Ishikari
, Japan
)
Abiko, Yoshihiro
( Health Sciences University of Hokkaido
, Sapporo
, Japan
)
Uehara, Osamu
( Health Sciences University of Hokkaido
, Ishikari-Tobetu
, Hokkaido
, Japan
)
Harada, Fumiya
( Health Sciences University of Hokkaido
, Sapporo
, Hokkaido
, Japan
)
Takai, Rie
( Health Sciences University of Hokkaido
, Ishikari-Tobetsu
, Hokkaido
, Japan
)
Hiraki, Daichi
( Health Sciences University of Hokkaido
, Sapporo
, Japan
)
Shimizu, Aya
( Health Sciences University of Hokkaido
, Ishikari-Tobetsu
, Japan
)
Toraya, Seiko
( Health Sciences University of Hokkaido
, Ishikari
, Japan
)
Adhikari, Bhoj
( Health Sciences University of Hokkaido
, Ishikari-Tobetsu
, Hokkaido
, Japan
)
Neopane, Puja
( Health Sciences University of Hokkaido
, Ishikari
, Japan
)