IADR Abstract Archives
Antibiofilm Effect of Chitosan-coated Iron Oxide Nanoparticles as Chlorhexidine Carrier
Objectives: This study aimed to synthesize a new chlorhexidine(CHX)-carrier nanosystem based on iron oxide magnetic nanoparticles (IONPs) and chitosan (CS), as well as to evaluate its effect on pre-formed biofilms of Candida albicans and Streptococcus mutans in mixed cultures.
Methods: The IONPs-CS-CHX nanosystem was prepared by loading of CHX on IONPs coated with CS, and characterized by X-ray diffraction (XRD), dynamic light scattering (DLS) and transmission electron microscopy (TEM). After, 24-h biofilms were formed in 96-well plates and treated for 24 h with the nanosystem at 39 (IONPs-CS-CHX39) and 78 µg/ml (IONPs-CS-CHX78). Antibiofilm effect was evaluated by quantification of cultivable cells, total biomass and metabolic activity. Appropriate controls were included in all analyzes. Data were analyzed by one-way ANOVA and Holm-Sidak’s post-hoc test (α = 0.05).
Results: XRD patterns of IONPs and IONPs-CS-CHX showed typical peaks of face-centered cubic system, characteristics of magnetite (Fe3O4) with a spinel structure. TEM and DLS confirmed the formation of the nanosystem with average size lower than 50 nm. For C. albicans, only IONPs-CS-CHX39 and IONPs-CS-CHX78 significantly reduced the number of cells when compared to the negative control (NC). For S. mutans, CHX, IONPs-CS-CHX39 and IONPs-CS-CHX78 were very effective in reducing the number of cells, attaining decreases ranging from 3.06- to 5.85-log10. Only IONPs-CS-CHX78 was able to significantly reduce the biomass in comparison to the NC (44%). Metabolic activity was significantly decreased after treatment with CHX (89.7%) and IONPs-CS-CHX78 (94.4%).
Conclusions: IONPs-CS-CHX78 showed similar or superior antibiofilm effect when compared with CHX alone. These findings highlight the benefits of this nanotechnology-based therapy, which may be useful in the treatment of biofilm-associated oral diseases.
Methods: The IONPs-CS-CHX nanosystem was prepared by loading of CHX on IONPs coated with CS, and characterized by X-ray diffraction (XRD), dynamic light scattering (DLS) and transmission electron microscopy (TEM). After, 24-h biofilms were formed in 96-well plates and treated for 24 h with the nanosystem at 39 (IONPs-CS-CHX39) and 78 µg/ml (IONPs-CS-CHX78). Antibiofilm effect was evaluated by quantification of cultivable cells, total biomass and metabolic activity. Appropriate controls were included in all analyzes. Data were analyzed by one-way ANOVA and Holm-Sidak’s post-hoc test (α = 0.05).
Results: XRD patterns of IONPs and IONPs-CS-CHX showed typical peaks of face-centered cubic system, characteristics of magnetite (Fe3O4) with a spinel structure. TEM and DLS confirmed the formation of the nanosystem with average size lower than 50 nm. For C. albicans, only IONPs-CS-CHX39 and IONPs-CS-CHX78 significantly reduced the number of cells when compared to the negative control (NC). For S. mutans, CHX, IONPs-CS-CHX39 and IONPs-CS-CHX78 were very effective in reducing the number of cells, attaining decreases ranging from 3.06- to 5.85-log10. Only IONPs-CS-CHX78 was able to significantly reduce the biomass in comparison to the NC (44%). Metabolic activity was significantly decreased after treatment with CHX (89.7%) and IONPs-CS-CHX78 (94.4%).
Conclusions: IONPs-CS-CHX78 showed similar or superior antibiofilm effect when compared with CHX alone. These findings highlight the benefits of this nanotechnology-based therapy, which may be useful in the treatment of biofilm-associated oral diseases.