Antibacterial Effect of a Novel CPC-Clay Containing Toothpaste

Objectives: To assess the antibacterial efficacy of cetylpyridinium chloride-clay (CPC-Clay) containing toothpaste via in-vitro and in-vivo studies
Methods: In-vitro performance was assessed through hydroxyapatite discs (n=10) treated with a test toothpaste (containing1.5% CPC-clay) in well plates for 3 mins, then coated with sterile human saliva, and incubated in a S.mutans (ATCC 25175) suspension for 12 hours. Bacterium bound to the HAP disc was measured as total viable count (TVC). The counts were converted into log₁₀ values to equalize variance in the data and the data was analysed using Tukey-Kramer test. In-vivo performance was evaluated in a randomised, double blind, crossover clinical trial (n=60) examining the effect of 1.5% CPC-Clay toothpaste and standard fluoride toothpaste on plaque growth over 24hours post brushing. Plaque was scored using modified gingival marginal plaque index (MGMPI). ANCOVA model was used to analyse the post-brushing plaque scores with product and visit as fixed effect, and baseline scores as covariate. Tukey adjustment was used to account for the multiple comparisons.
Results: Results are shown in the tables.
Conclusions: Toothpaste with 1.5% CPC-Clay has comparable antibacterial activity to a marketed toothpaste with 0.3% Triclosan at 12 hours timepoint. The clinical study established that toothpaste with 1.5% CPC-Clay gave significantly lower plaque growth compared to a standard fluoride toothpaste over 24 hours.