IADR Abstract Archives

Dysbacteriosis Leading to Impaired Dentin Reparation of Murine Incisor

Objectives: The purpose of this study was to figure out the effect of dysbacteriosis on reparative dentin formation as well as the biological characteristics of dental pulp stem cells (DPSCs).
Methods: The microflora imbalance mouse model was established by antibiotic treatment and divided into 3 groups: (A) mice treated with oral administration of antibiotics (AbT); (B) specific-pathogen free (SPF); (C) after treated with antibiotics, mice were co-housed with SPF mice and conventionally raised (ConvD). Lower right incisor of each mouse was mechanically ground for 2 mm, and the height difference between two incisors was recorded. Samples were taken after 7 days, and the characteristics of dental pulp and reparative dentin formation were analyzed by HE staining. The infiltration of inflammatory cells and the expression of cytokines were explored by immunohistochemistry. DPSCs were isolated from the intact incisors of three groups. The proliferative and odontogenic ability of DPSCs were compared between three groups by CFU, CCK-8 and Alizarin Red staining.
Results: The incisors of AbT group repaired significantly slower than ConvD and SPF groups, while there was no significant difference between ConvD and SPF group. Immunohistochemical analysis showed that the expression of inflammatory cytokines such as TNF-α and IL-4, and TLR-4 were down-regulated, and the number of F4/80+ dendritic cells was reduced in the dental pulp of AbT incisors. Compared to SPF group, there were less colony units and mineral nodes formed in DPSCs of AbT group which were rescued in ConvD group. There was no significant difference in DPSC proliferative ability between the three groups.
Conclusions: Dysbacteriosis caused by antibiotics led to impaired dental pulp recovery and slower growth rate in mice incisor, which may be associated with the alteration of local immunity and biological properties of DPSCs.
IADR/PER General Session
2018 IADR/PER General Session (London, England)
London, England
2018
0162
Pulp Biology & Regeneration Research
  • Zhao, Yuming  ( Peking University Hospital of Stomatology , Beijing , China )
  • Su, Shenping  ( Peking University Hospital of Stomatology , Beijing , China )
  • Xiao, E  ( Peking University, Hospital of Stomatology , Beijing , China )
  • the Capital Featured Clinical Application Research Project of Beijing Municipal Science & Technology Commission (Zl51100004015093)
    NONE
    Oral Session
    Dentin Function & Regeneration
    Wednesday, 07/25/2018 , 09:30AM - 11:00AM