IADR Abstract Archives
Comparison Between Histomorphometric and Micro-CT Analysis for Bone Evaluation After GBR: Preliminary Results
Objectives: Background: Micro-CT is a radiological instrument that allows to have a high definition volumetric 3D analysis of bone tissue. This method seems to be more accurate and to give more information about bone morphology in comparison to histomorphometric analysis, that is a sectional 2D analysis. However, the most studies about Guided bone regeneration (GBR) reported results of histological analysis. The aim of this report is to compare micro-CT analysis and histomorphometric one of newly-formed bone after GBR.
Methods: Material and Methods: All patients were randomly treated for alveolar ridge deficiencies using GBR with Ti-reinforced PTFE membranes or titanium mesh and collagen membranes. After 9 months of submerged healing, bone tissue biopsies were collected from augmented sites. Bone volume (B.V), material volume (Mat.V), soft tissue volume (St.V), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were measured using micro-CT. The following histomorphometric parameters were considered: bone area (B.Ar), material area (Mat.Ar), and soft tissue area (St.Ar). A novel histological parameter was introduced to describe the inner bone structure. Correlations between related parameters were performed with Spermann’s test. Statistical significance was set at a = 0.05.
Results: Results: Only 18 samples out of 40 patients were collected up to date. The following micro-CT parameters were observed: 30%, 9%, 61%, 0,1%, 2,0%, and 0,2% for B.V, Mat.V, St.V, Tb.Th, Tb.N, and Tb.Sp in PTFE group; similarly, 30%, 12%, 58%, 0,1%, 2,5%, and 0,2% in titanium mesh group. Histomorphometric parameters were: 40%, 9%, and 51% for PTFE group and 41%, 10%, and 49% for titanium mesh group. High correlation levels were noted between micro-CT and histological analyses for all parameters. Bone structure index resulted to be related to trabecular parameters.
Conclusions: Conclusions: The preliminary results of this study suggested a correlation between micro-CT and histomorphometric parameters. Micro-CT resulted in a more accurate and efficient analysis of bone tissue. Complete results and further studies are required to confirm the efficiency of bone structure index to describe the newly-formed bone.
Methods: Material and Methods: All patients were randomly treated for alveolar ridge deficiencies using GBR with Ti-reinforced PTFE membranes or titanium mesh and collagen membranes. After 9 months of submerged healing, bone tissue biopsies were collected from augmented sites. Bone volume (B.V), material volume (Mat.V), soft tissue volume (St.V), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) were measured using micro-CT. The following histomorphometric parameters were considered: bone area (B.Ar), material area (Mat.Ar), and soft tissue area (St.Ar). A novel histological parameter was introduced to describe the inner bone structure. Correlations between related parameters were performed with Spermann’s test. Statistical significance was set at a = 0.05.
Results: Results: Only 18 samples out of 40 patients were collected up to date. The following micro-CT parameters were observed: 30%, 9%, 61%, 0,1%, 2,0%, and 0,2% for B.V, Mat.V, St.V, Tb.Th, Tb.N, and Tb.Sp in PTFE group; similarly, 30%, 12%, 58%, 0,1%, 2,5%, and 0,2% in titanium mesh group. Histomorphometric parameters were: 40%, 9%, and 51% for PTFE group and 41%, 10%, and 49% for titanium mesh group. High correlation levels were noted between micro-CT and histological analyses for all parameters. Bone structure index resulted to be related to trabecular parameters.
Conclusions: Conclusions: The preliminary results of this study suggested a correlation between micro-CT and histomorphometric parameters. Micro-CT resulted in a more accurate and efficient analysis of bone tissue. Complete results and further studies are required to confirm the efficiency of bone structure index to describe the newly-formed bone.