Biosilicate^® can Minimize the Pulp Damage Caused by Dental Bleaching

Objectives: To evaluate the therapeutic effect of Biosilicate®-based gel (BS) on the dental pulp tissue of Wistar rat molars undergoing dental bleaching with hydrogen peroxide (H₂O₂).
Methods: The upper molars of 60 rats were randomly divided into the following groups: Ble: bleached (35%H₂O₂; 30 min); Ble-BS: bleached followed by single BS application (20min); BS-Ble: single BS application (20min) and then bleached; BS/7d-Ble: BS applications (20min) for 7 days, and then bleached; Ble+BS: blend of H₂O₂ with BS (1:1; 30min); and Control. After 2 and 30 days, the rats were killed and jaws processed for evaluation in hematoxylin-eosin. The data were subjected to Kruskal-Wallis and Dunn statistical test (P<.05).
Results: At 2 days, severe inflammation and necrosis were observed in occlusal third of the coronal pulp of Ble group, and severe inflammation in Ble-BS, both different from the Control (P<.05); the groups with application of BS before dental bleaching had moderate inflammation, without difference compared with other groups (P>.05); Ble+BS had moderate inflammation, and was different from Ble and Ble-BS groups (P<.05). In the middle third, the Ble and Ble-BS groups had severe and moderate inflammation, respectively (P>.05), both different from the Control (P<.05); BS-Ble, BS/7d-Ble and Ble+BS groups had mild inflammation, with significant difference compared with the Ble group (P<.05), and was similar to Control and Ble-BS groups (P>.05). In the cervical third, there was also differences among Ble and Ble-BS groups, with moderate and mild inflammation, compared with Control (P<.05); BS-Ble, BS/7d-Ble and Ble+BS groups had similar to Control and Ble-BS groups (P>.05), and different from the Ble group (P<.05). At 30 days, there was absence of inflammation in all groups (P>.05), and tertiary dentin in bleached groups.
Conclusions: Biosilicate® can minimize the pulp damage caused by dental bleaching