Immunohistochemical Assessment of MMPs After Using Adhesive on Human Teeth

Objectives: This study was conducted to evaluate the immunohistochemical expression of MMPs 9 and 13, also TIMP-3 after use XP Bond™ adhesive system (Dentsply) in human dentin, in different time intervals.
Methods: Deep cavities were made on the occlusal surface of twenty one human molars. The teeth were restored with the adhesive XP Bond™ and composite Z250 A3 (3M ESPE). After 01, 07, 14, 21, 30, 90 and 120 days, the teeth were extracted and prepared for immunohistochemical evaluation. The immunohistochemical staining was performed by the method of streptavidin-biotin optimized peroxidase with LSAB amplification system (Labeled StresAvidin Biotin is mouse primary antibodie - DAKO), using monoclonal antibodies anti-MMP-9, anti-MMP-13 and anti-TIMP-3 and diaminobenzidine as a chromogen.
Results: The expression of MMPs 9 an 13 was considered severe and moderate, respectively in first intervals studied, declining over time. However, the immunoreactivity of TIMP-3 was absent in dentin and weak in pulp first time intervals, decreasing totally absent in the subsequent intervals. About the location, MMP-9 dentinal expression was predominant in dentine tubule; the expression in the pulp was predominant in odontoblast layer. MMP-13 expression in dentin and in pulp was predominant in the pre-dentine region and odontoblastic layer, respectively. TIMP-3 expression was absent in dentine and had predominant location in pulp’s odontoblastic layer.
Conclusions:
Thus, as a conclusion, this study showed that the expression pattern of MMP-9, MMP-13 and TIMP-3 undergoes change in different time intervals studied, demonstrated intimate relationship with the progression of the inflammatory process. Increased TIMP-3 expression during the first time intervals can demonstrate a synchronous response to the increased expression of MMP in the same periods. The results showed low TIMP-3 activity in physiological processes dentin-pulp complex for the tested conditions and materials.