Comparison of Periodontopathogens and Detection of Tetracycline Resistance Genes by PCR

Objectives: To compare the periodontopathogens and to detect the tetracycline resistance genes by PCR among patients with different periodontal conditions.
Methods: Five subgingival samples from each periodontic cases which includes chronic periodontitis and aggressive periodontitis as well as healthy population were subjected to 16s rDNA analysis for P. gingivalis, T. denticola, T. forsythia, A. actinomycetemcomitans, P. intermedia and Eikenella corrodens followed by the detection of tetracycline resistance genes by PCR as described elsewhere. The amplicons were resolved in 4% agarose gel electrophoresis and findings were compared.
Results: In our study, T. forsythia was the most frequently detected pathogen in 5/5 (100%) chronic periodontitis and 4/5 (80%) aggressive periodontitis patients. A. actinomycetemcomitans was the least detected pathogen as it detected only one sample of chronic periodontitis case. Upon subjected them to tetracycline resistance gene (TetM gene), all were negative.
Conclusions: T. forsythia and A. actinomycetemcomitans were the most frequently and least detected periodontic pathogens respectively. Tetracycline resistance was not observed in our isolates. This study reveals the knowledge on the prevalence of periopathogens and tetracycline resistance gene in our region.