Ion Release and Cytotoxicity of Acrylic Resins Incorporated With Nanomaterial
Objectives: Release of ions and cytotoxicity of autopolymerizing (AP) and heat-polymerizing (HP) acrylic resins incorporated with nanostructured silver vanadate decorated with AgNPS (AgVO3) in different percentages: 0%, 0.5%, 1%, 2.5%, 5% and 10% were analyzed.
Methods: The samples were immersed in culture medium for 30 days. The release of silver ions (Ag) and vanadium (V) was evaluated by mass spectrometry with inductively coupled plasma (ICP-MS) (n=9) and the cell viability of fibroblasts L929 by the colorimetric assay to measure the reduction of MTT (3-[4,5-Dimethylthiazol- 2yl]-2,5-diphenyltetrazolium bromide) (n=12). The data were analyzed by ANOVA and Tukey's test (α=0.05).
Results: The control group (0%) had low concentrations of Ag and V ions, different from the others (P<0.0001). The HP resin released significant amounts of Ag starting at 2.5% (P<0.0001) and AP resin starting at 5% (P<0.0001). For V, in both resins, there was a more release at concentrations of 2.5, 5 and 10% of AgVO3 (P<0.0001). All groups showed a reduction for L929 cell viability when compared with the control (100%), including the control samples of the resins, without AgVO3 (P<0.0001). In comparison with the control resins for HP, a significant reduction in the metabolism of cells occurred starting at 2.5% and for AP at 5% (P<0.0001).
Conclusions: Significant amounts of Ag and V ions were released from acrylic resins with higher concentrations of AgVO3, indicating that Ag was probably the main ion responsible for reducing cell viability of L929.