IADR Abstract Archives
Red-blue Light Treatment on Candida albicans Biofilm Development
Objectives: The use of incoherent light has been studied extensively in inhibiting the development of biofilms in both bacterial and fungal species. While incoherent light’s effects have been studied with and without the presence of a photosensitizer in Streptococcus mutans, there is currently no literature testing the light’s effectiveness on Candida albicans without a photosensitizer. This study examines and compares the effects of blue and red light of equal energy densities on C. albicans biofilm formation.
Methods: Biofilms of Candida albicans SN425 were formed on 24-well tissue culture plates. The biofilms were exposed periodically to red light (LumaCare; 630 nm) and blue light (LumaCare; 420 nm): Once after the adhesion phase (90 min), and twice daily for an additional two days. The distance between the light and the sample was 0.5 cm; the energy density was 72 Jcm-2; with exposure times for blue light being 12 minutes 56 sec and 27.3 sec for the red light (equal energy densities). Positive and negative controls were 0.2% chlorhexidine and 0.89% NaCl. Dry weight and colony forming units (CFUs) of the exposed biofilms were measured.
Results: Both red and blue light resulted in reduced colony densities per dry weight (cfu/mg) when compared to the negative control (6.21x10-5 cfu/mg). Red light resulted in a colony density of 3.76x10-5 cfu/mg and blue resulted in 1.44x10-5 cfu/mg.
Conclusions: When comparing incoherent light of equivalent energy densities (72 Jcm-2), blue light was consistently shown to be superior to redlight in the inhibition of biofilm development of C. albicans. Blue light treatment achieved results comparable to that of 0.2% chlohexidine. Blue-light treatment appears to be a promising and viable option in preventing and combating the formation of C. albicans.
Methods: Biofilms of Candida albicans SN425 were formed on 24-well tissue culture plates. The biofilms were exposed periodically to red light (LumaCare; 630 nm) and blue light (LumaCare; 420 nm): Once after the adhesion phase (90 min), and twice daily for an additional two days. The distance between the light and the sample was 0.5 cm; the energy density was 72 Jcm-2; with exposure times for blue light being 12 minutes 56 sec and 27.3 sec for the red light (equal energy densities). Positive and negative controls were 0.2% chlorhexidine and 0.89% NaCl. Dry weight and colony forming units (CFUs) of the exposed biofilms were measured.
Results: Both red and blue light resulted in reduced colony densities per dry weight (cfu/mg) when compared to the negative control (6.21x10-5 cfu/mg). Red light resulted in a colony density of 3.76x10-5 cfu/mg and blue resulted in 1.44x10-5 cfu/mg.
Conclusions: When comparing incoherent light of equivalent energy densities (72 Jcm-2), blue light was consistently shown to be superior to redlight in the inhibition of biofilm development of C. albicans. Blue light treatment achieved results comparable to that of 0.2% chlohexidine. Blue-light treatment appears to be a promising and viable option in preventing and combating the formation of C. albicans.