Effects of Fluorohydroxyapatite Gel on Proliferation and Differentiation of HDPSCs

Objectives: Objective: To investigate the effects of a new remineralization fluorohydroxyapatite gel on proliferation and differentiation of human dental pulp cells.
Methods: Methods: HDPSCs were harvested from impacted third molars of healthy adult individuals using enzyme digestion, expanded and cultured. The hDPSCs were cultured in DMEM medium containing Fluorohydroxyapatite gel（FHA）eluate for 7 days. Cell proliferation was assessed by CCK-8 cell proliferation kit on days 1, 3, 5 and 7. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to examine relative expression of DSPP, DMP-1, OCN, Runx2 and ALP. Alizarin red staining was performed on day 7 and 14.
Results: Results: The proliferation of hDPSCs in FHA gel eluate was higher than negative control group（P>0.05）. The expression of DSPP, DMP-1, OCN and Runx2 genes were significantly up-regulated compared with negative control group on day 3(P<0.05), and on day 7(P>0.05). Alizarin red staining showed that hDPSCs in FHA gel eluate formed much more calcium nodules than negative control group (P<0.05).
Conclusions: Conclusion: FHA gel has good biocompatibility to hDPSCs, but can promote the odonto and osteogenic differentiaion of hDPSCs.