IADR Abstract Archives
A Novel Point-of-care Test for Active Matrix Metalloproteinase-8 (aMMP-8) as a Predictor of Periodontal Disease in Diabetes Patients
Objectives: The detection of aMMP-8 (Collagenase-2) in saliva is a promising method for early detection of progressive periodontal tissue destruction especially for patients with diabetes belonging to a risk group. The aim of this clinical study was to determine the sensitivity and specificity of a point-of-care test Periosafe®Pro to detect periodontitis in patients without knowledge of illness.
Methods: Concentrations of aMMP-8 were analysed from salivary specimens and sample of sulcus fluid from two pockets of 98 individuals suffering from diabetes I and II (mean age 45,9y). The saliva test, a lateral flow immunochromatographic assay (ICGA), was processed according to the manufacturers instructions and the results (positive signals for aMMP-8) were visually detected. The sulcus fluid samples were also qualitatively examined by a laboratory. Subsequently, a periodontal status was measured without knowing MMP-8 results and patients were categorized into “non-periodontitis” and “periodontitis” (and further subdivided into different periodontitis forms).
Results: aMMP-8 detection by ICGA reached a sensitivity of 66.18% and a specificity of 56.67% to detect periodontitis. The detection of individuals with periodontal disease was significant (chi-squared test p=0.034).
Conclusions: Identification of previously unknown periodontitis was significantly better by using the Periosafe®Pro than by chance although there is room for improvement of the screening characteristics, given the observed sensitivity and specificity of the Periosafe®Pro test. However, considering the relative young mean age and the fact that patients were not conscious of any periodontal disease, the - objectively - low sensitivity and specificity offers the opportunity to detect patients suffering from undetected periodontitis, anyway.
Methods: Concentrations of aMMP-8 were analysed from salivary specimens and sample of sulcus fluid from two pockets of 98 individuals suffering from diabetes I and II (mean age 45,9y). The saliva test, a lateral flow immunochromatographic assay (ICGA), was processed according to the manufacturers instructions and the results (positive signals for aMMP-8) were visually detected. The sulcus fluid samples were also qualitatively examined by a laboratory. Subsequently, a periodontal status was measured without knowing MMP-8 results and patients were categorized into “non-periodontitis” and “periodontitis” (and further subdivided into different periodontitis forms).
Results: aMMP-8 detection by ICGA reached a sensitivity of 66.18% and a specificity of 56.67% to detect periodontitis. The detection of individuals with periodontal disease was significant (chi-squared test p=0.034).
Conclusions: Identification of previously unknown periodontitis was significantly better by using the Periosafe®Pro than by chance although there is room for improvement of the screening characteristics, given the observed sensitivity and specificity of the Periosafe®Pro test. However, considering the relative young mean age and the fact that patients were not conscious of any periodontal disease, the - objectively - low sensitivity and specificity offers the opportunity to detect patients suffering from undetected periodontitis, anyway.