Cytotoxicity of Ethanolic leaf Extract of Caralluma fimbriata on Oral Cancer Cell Line

Objectives: The present study was carried out to assess the in vitro anticancer effect of ethanolic leaf extract of Carulloma fimbriata on oral epidermoid cancer cells utilizing cell viability.
Methods: The antiproliferative and cytotoxicity study was carried out on KB cell line by MTT assay using three different concentrations of the ethanolic leaf extract of Carulloma fimbriata (100, 250 and 500µg/ml). The IC₅₀ value of the extract was also calculated. The cell death inducing effect of the extract was determined by morphological observation under inverted microscope. The in vitro assay data signify mean ± standard deviation of triplicates.
Results: The extract showed dose dependent cytotoxic effect against oral cancer cell line. The cell viability showed at 500µg/ml was 28.47% compared to the standard 21.87 % and the cytotoxicity produced was 71.52% compared to standard Cyclophosphamide 78.12%.
Conclusions: The extract showed significant decrease in cell viability compared to standard Cyclophosphamide. This Decrease in cell viability indicates the antiproliferative potential of Carulloma fimbriata extract against KB oral carcinoma cells and can be considered as a future candidate for the development of oral cancer chemotherapeutic agent.