IADR Abstract Archives
Xylene With Kerosene as a Clearing Agent: a Comparative Study
Objectives: 1) To evaluate the effectiveness of mixture of xylene and kerosene as a clearing agent.
2) To assess the ideal percentage of xylene and kerosene required to be used as a clearing agent in histopathology.
Methods: Samples of tissues from specimens were collected from the archives of oral maxillofacial pathology of Saveetha dental college (n=18, 6 samples under each group) (SRB/STUG12/45)
Xylene and kerosene were used in the following concentrations;
Group A: 70% xylene:30% kerosene (solvent 1)
Group B: 50% xylene:50% kerosene (solvent 2)
Group C: 100% xylene (solvent 3)
The sections obtained were processed and subjected to hematoxylin and eosin staining procedure. Groups A, B and C were cleared in solvents 1,2 and 3 respectively. Duration for clearing was one hour ,half an hour and a few seconds for group A,B and C respectively. Histological procedure were carried out under same laboratory conditions and viewed using standard criteria under a light microscope.
Results: 70% xylene with 30% kerosene used as a clearing agent showed to have good cellular clarity and uniform staining. However with 50% xylene and 50% kerosene a difference in the cellular architecture was observed. Thus using 70% xylene with 30% kerosene reduces the concentration of plain xylene thereby reducing its hazardous nature with the maintenance of cellular architecture as compared to using 100% xylene.
Conclusions: It can be inferred from this investigation that a mixture of xylene and kerosene could be employed as a clearing agent. Although kerosene has a flammable nature when combined with xylene its hazardous properties are reduced and can be used on a long term basis. This novel method which combines the use of xylene and kerosene as a clearing agent is the first of its kind in oral pathology.
2) To assess the ideal percentage of xylene and kerosene required to be used as a clearing agent in histopathology.
Methods: Samples of tissues from specimens were collected from the archives of oral maxillofacial pathology of Saveetha dental college (n=18, 6 samples under each group) (SRB/STUG12/45)
Xylene and kerosene were used in the following concentrations;
Group A: 70% xylene:30% kerosene (solvent 1)
Group B: 50% xylene:50% kerosene (solvent 2)
Group C: 100% xylene (solvent 3)
The sections obtained were processed and subjected to hematoxylin and eosin staining procedure. Groups A, B and C were cleared in solvents 1,2 and 3 respectively. Duration for clearing was one hour ,half an hour and a few seconds for group A,B and C respectively. Histological procedure were carried out under same laboratory conditions and viewed using standard criteria under a light microscope.
Results: 70% xylene with 30% kerosene used as a clearing agent showed to have good cellular clarity and uniform staining. However with 50% xylene and 50% kerosene a difference in the cellular architecture was observed. Thus using 70% xylene with 30% kerosene reduces the concentration of plain xylene thereby reducing its hazardous nature with the maintenance of cellular architecture as compared to using 100% xylene.
Conclusions: It can be inferred from this investigation that a mixture of xylene and kerosene could be employed as a clearing agent. Although kerosene has a flammable nature when combined with xylene its hazardous properties are reduced and can be used on a long term basis. This novel method which combines the use of xylene and kerosene as a clearing agent is the first of its kind in oral pathology.