IADR Abstract Archives
BMP-2 Generate More Bone in Critical-size-bone-defect Compare to PRP and L-PRF
Objectives: Bone Morphogenic Protien-2 (BMP-2) have been demonstrated to play an important role in bone and cartilage healing. Platelets-Rich-Plasma (PRP) and Leukocyte-Platelets-Rich-Fibrin (L-PRF), on the other hand, were shown to improve wounds healing. In our study we compared the effect of these materials on bone formation in critical size bone defect of mice.
Methods: 36 mice were divided into 6 groups (n=6), negative control, SurgiFoam only, SurgiFoam + low concentration (0.3 µg) BMP-2, surgiFoam + High concentration (1.4 µg) BMP2, PRP, and L-PRF. The 5mm calvarial bone defect was generated at the middle of skull of the mouse using trephine. The 5mm defects, then, were allowed to heal for six weeks. After six weeks, the bone defect was collected and samples were analyzed for bone formation macroscopically and histologically; utilizing the X-rays, H&E staining, and Alkaline Phosphatase (ALP).
Results: The remaining surface area were calculated, in square mm, by the X-ray shows that BMP-2 were able to almost completely close the defect compared to the other groups. The Histological analysis also confirm the same findings. in H&E stained samples, the defect was appeared to be closed. As well as, the ALP staining showed higher ALP activity in both BMP-2 groups.
Conclusions: All in all, our results show that 0.3µg and 1.4µg of BMP2 was able to produce an almost complete healing of the critical size defect (90-95%) after six weeks. However, SurgiFoam, PRP and L-PRF was not able to close more than 60% of the original defect. Our study support that BMP-2 is more powerful for bone regeneration than SurgiFoam, PRP and L-PRF.
Methods: 36 mice were divided into 6 groups (n=6), negative control, SurgiFoam only, SurgiFoam + low concentration (0.3 µg) BMP-2, surgiFoam + High concentration (1.4 µg) BMP2, PRP, and L-PRF. The 5mm calvarial bone defect was generated at the middle of skull of the mouse using trephine. The 5mm defects, then, were allowed to heal for six weeks. After six weeks, the bone defect was collected and samples were analyzed for bone formation macroscopically and histologically; utilizing the X-rays, H&E staining, and Alkaline Phosphatase (ALP).
Results: The remaining surface area were calculated, in square mm, by the X-ray shows that BMP-2 were able to almost completely close the defect compared to the other groups. The Histological analysis also confirm the same findings. in H&E stained samples, the defect was appeared to be closed. As well as, the ALP staining showed higher ALP activity in both BMP-2 groups.
Conclusions: All in all, our results show that 0.3µg and 1.4µg of BMP2 was able to produce an almost complete healing of the critical size defect (90-95%) after six weeks. However, SurgiFoam, PRP and L-PRF was not able to close more than 60% of the original defect. Our study support that BMP-2 is more powerful for bone regeneration than SurgiFoam, PRP and L-PRF.