Could P.gingivalis Infection Modulate Th-17 Cytokines in Delivery Adverse-outcomes?

Objectives: The aim of this study was to evaluate th17 profile cytokines levels in placental samples from women with delivery adverse outcomes
Methods: Twenty seven pregnancy women from Hospital Simón Bolivar (Bogotá, Colombia) were included in this study, which were dived in three groups: Women which didn’t have any adverse outcome as control group (n=17), women with P. gingivalis intra-amniotic infection (n=5) and women with intra-amniotic infection related with medical relevance microorganisms (n=5). It was made clinical and periodontal evaluation and placental samples were taken after delivery. All samples were processed to determine soluble levels of IL17A, IL17F, IL21, IL23, TNF alpha, IL1 beta and IL6 by flow cytometric bead array. The statistical analysis was made using IBM-SPSS V23 for Windows. This study was approved by the Ethics Committee of the Universidad El Bosque (Bogotá, Colombia)


Results: The average of age according group were 21.2±3.1, 24.6±4.6 and 22.2±3.1 for control group, P. gingivalis intra-amniotic infection group and intra-amniotic infection related with medical relevance microorganisms group respectively. 60% of women in P. gingivalis intra-amniotic group had periodontitis by CDC criteria, which also had P. gingivalis in periodontal plaque (p<0.05 by Chi square test). It was observed significant differences for IL17A, IL17F, IL21, IL23 and TNF alpha cytokines among groups (p<0.05 by Kruskall Wallis test), showing significant reductions of this levels in P gingivalis group when were compared with control group (p< 0,005 by U Mann Whitney test). Control group did not show any difference with intra-amniotic infection related with medical relevance microorganisms group.
Conclusions: The evidence has been showing the relationship between periodontitis with adverse outcomes of pregnancy, however this is the first study that shows how P. gingivalis intra-amniotic infection can induce a significant reduction of Th17 cytokines in placental samples.