Semi-Synthetic Glycoaminoglycan Ethers Inhibit Ca9-22 Cell Invasion Induced by CSE

Objectives: Oral squamous cell carcinoma is a common disease affecting approximately thirty thousand people in the United States each year. One of the major causes of such cancer is exposure to tobacco smoke.The mechanistic effects to of secondhand smoke in the development of gingival carcinoma are limited and still warrant investigation. SAGEs are inflammation modulating sulfated polysaccharides known to inhibit RAGE receptors. Our objective is to use SAGE as a modulating factor of Ca9-22 cell invasion during CSE treatment.
Methods: Ca9-22 cells were cultured using RPMI supplemented with 10% of fetal calf serum. Cells were cultured in the presence of 0.05% CSE with or without SAGEs (50ug/mL). Real invasions were performed.
Results: Treatment of cells with CSE: 1) showed increased invasion by 1.8 fold (p<0.0001), and 2) SAGEs addition showed a reduction by 1.8 fold (p<0.0003). This reduction brought the invasion levels to control levels.
Conclusions: We conclude that SAGEs are sufficient to reduced the increased cell invasion in gingival cancer cells induced by CSE. This result could be useful in providing avenues that could explain the role of second hand smoke in the development of oral squamous cell carcinoma.