Antimicrobial Action of Denture Cleansers: A Preliminary Clinical Trial
Objectives: This preliminary study reports a cross-over trial about the efficacy of three denture cleansers (sodium hypochlorite, alkaline peroxides and Rininus communis solution) in terms of antimicrobial action against Candida spp.
Methods: Twelve denture wearers were instructed to brush their dentures (specific brush and liquid soap) three times a day and to soak them (20 minutes/7 days) in the following solutions: (SH) 0.20% Sodium Hypochlorite, (E) Efferdent Power Clean Crystals, (RC) 6,5% Castor oil solution, (Control) Saline. Furthermore, half of the sample (n=6) was instructed to soak their brushes in the same solutions of the denture. After each product use, the internal surfaces biofilm of maxillary dentures was collected (brushed with saline solution) and the obtained suspension was diluted (100 to 10-3) and seeded (50 µL) in CHROMagar for Candida spp.. After incubation, CFU/mL values were calculated. Data were analyzed by Friedman (α=0.05) and Wilcoxon tests and corrected by Bonferroni (α=0.005).
Results: There was no difference between toothbrushes immersion or non-immersion (p>0.005). SH (Mean Rank – MR=2.42) and E (MR=2.46) showed antimicrobial action for Candida spp. and RC (MR=2.62) showed similar results to C (MR=3.17) and Baseline (MR=4,33).
Conclusions: 0.20% Sodium hypochlorite and Efferdent showed effect on reducing Candida spp. colonies. Toothbrushes immersion on solutions did not influence antimicrobial action results.