IADR Abstract Archives
Homogenous Inhabitance of Cells Into Critical Sized Defect Model Scaffold
Objectives: Regenerative medicine and tissue engineering using scaffolds have beneficial influences on the repair of bony defects. The hydrodynamics is one of the limelight techniques for optimizing cellular activity for bone tissue engineering applications. However, imbalance of cellular proliferation and differentiation between the outer and center of the scaffold result in cell death and incomplete bone growth after implantation in the body. Hence, the feasibility of penetration and dwelling of cells into a critical sized defect model scaffold via hydrodynamics induced by rocking motion was investigated.
Methods: A 1x1x2cm cuboidal hydroxyapatite scaffolds were prepared. The 2cm length can be considered as a critical-sized segmental defect. MC3T3-E1 cells were seeded into the scaffolds. Customized rocking culture plates were designed. Three experimental groups were simulated by rocking up and down ± 15° at 10, 20, and 30 cycle/min, 2 times/day for 10 minutes. The rocking culture was applied after three days static culture for the stabilization of cells. After 3, 6, 9, 12, and 15 days, homogeneity and viability of cell population was investigated using MTT assay, LIVE/DEAD assays, histology, and SEM. Both sides and center regions of scaffolds were compared.
Results: MTT assay were shown that the groups at static, 10 and 20 cycle/min, the cell viability in the center region of scaffold were lower than that of the side parts at day 7. However, after 7 days with 30 cycle/min, the cell viabilities in the center region of scaffold were higher than in the side parts. Furthermore, after 7 days with 30 cycle/min rocking demonstrated less imbalance of cell proliferation throughout the scaffold than other conditions.
Conclusions: The cellular responses such as penetration, proliferation, live/dead, and differentiation could be controlled by scaffold structure as well as culture conditions. In advance, the bone-like scaffold with rocking motion could apply to tissue engineering massive skeletal defects.
Methods: A 1x1x2cm cuboidal hydroxyapatite scaffolds were prepared. The 2cm length can be considered as a critical-sized segmental defect. MC3T3-E1 cells were seeded into the scaffolds. Customized rocking culture plates were designed. Three experimental groups were simulated by rocking up and down ± 15° at 10, 20, and 30 cycle/min, 2 times/day for 10 minutes. The rocking culture was applied after three days static culture for the stabilization of cells. After 3, 6, 9, 12, and 15 days, homogeneity and viability of cell population was investigated using MTT assay, LIVE/DEAD assays, histology, and SEM. Both sides and center regions of scaffolds were compared.
Results: MTT assay were shown that the groups at static, 10 and 20 cycle/min, the cell viability in the center region of scaffold were lower than that of the side parts at day 7. However, after 7 days with 30 cycle/min, the cell viabilities in the center region of scaffold were higher than in the side parts. Furthermore, after 7 days with 30 cycle/min rocking demonstrated less imbalance of cell proliferation throughout the scaffold than other conditions.
Conclusions: The cellular responses such as penetration, proliferation, live/dead, and differentiation could be controlled by scaffold structure as well as culture conditions. In advance, the bone-like scaffold with rocking motion could apply to tissue engineering massive skeletal defects.