Genetic Response Between Angiogenesis and Osteogenesis Markers in Mouse Mandibular Bone During Development

Objectives: Angiogenesis and osteogenesis markers are an important factor in formation of mandible. However, it has many other functions in addition to the regulation of mandible. We try to clearly the relationship between angiogenesis and osteogenesis markers are an important factor in formation of mandible. However, these markers in terminating the differentiation of the mandible. Therefore we try to investigate the correlation between these markers during the development of the mandible.
Methods: We analyzed the expression and localization of VEGF and osteopontin by in-situ hybridization in the developing mouse mandible during embryonic days 12.5 (E12.5), E14.5, E17.5, and E18.5 day 0, day 1 and days 5. We also detected the mRNA abundance of mandible with various markers from embryonic and early postnatal stages by real-time RT-PCR. Principal component analysis (PCA) was performed for mouse mandible in different postnatal stages and after birth and the expression of the following 8 mRNA markers (CD31, LYVE-1, VEGF, collagen I, collagen II, osteopontin, osteocalcin, MMP-2).
Results: The antisense probe for VEGF and osteopontin was detected in mesenchymal cells surrounding around MC and bone matrices area from E17.5, and clearly localization was seen at E18.5 in the embryonic mouse mandible by in situ hybridization. However, collgen II mRNA abundance pattern is resemble to that of osteopontin in compared with other markers (p< 0.05). The two principal components significantly explained 74.2% (component 1, 46.0%; component 2, 28.2%) of the information in the data set in the mandible. Cluster analyses identified the following distinct clusters for mRNA abundance in the mandible: cluster 1, E12.5; cluster 2, E 14.5, E17.5, day 0, and day; cluster 3, days 5.
Conclusions: These data suggested that angiogenesis and osteogenesis markers were effected by development stages after or before birth in morphogenesis. VEGF also affects the other morphological markers in these specific stages terminating the differentiation in mouse mandibular development.