IADR Abstract Archives
Dentine Permeability by an Experimental Formulation Containing Stannous Fluoride
Objectives: Hydraulic Conductance (HC) has been used extensively to assess the efficacy of occlusion based desensitising agents in vitro .In this work, three HC studies were performed to compare an experimental dentifrice to a commercially available sensitivity dentifrice (both containing 0.454%w/w SnF2). Studies were run over 2-4 days, with/without Acid Challenges Conditions (ACC).
Methods: HC1&2 involving 48hr HC with/without ACC, respectively, and HC3 involved 4 day HC with ACC. Test Dentifrices were an Experimental Dentifrice (ExpSnF2) and Sensodyne® Repair and Protect (SRP). Human dentine disks (n=10/dentifrice) with patent tubules were placed into a split chamber device connected to an Earle’s solution fluid reservoir. Hydrostatic pressure (1psi) was applied to the underside of the dentine disk and measurement of baseline flow rate recorded. The dentine was treated with dentifrice, the reduced flow rate measured and flow rate percentage reduction from baseline calculated. The dentine and chamber were stored in Artificial Saliva at 37oC for 24hrs post-treatment, and Δ% calculated per treatment. ACC was performed with dietary acid immediately prior to the final measurement for the HC2, and before and after the final treatment in HC3.
Results: Statistical analysis of the results showed that both dentifrices reduced Dentine Permeability (DP) effectively. Treatment differences for Δ% (dΔ%= ExpSnF2(Δ%)-SRP(Δ%)) in each study were: HC1 dΔ%[Initial=22.04%, 24hrs=54.75%, 48hrs=49.39%]; HC2 dΔ%[Initial: 20.37%, 24hrs: 20.73%, 48hrs: 36.99%); HC3 dΔ%[Initial: 23.07%, Day 1: 17.75%, Day 2: 21.91%, Day 3: 19.05%, Day 4: 30.22%].The ExpSnF2 reduced DP to a greater extent than SRP with statistically significant reductions in HC1, 2 and 3 over all time points (p<0.05).
Conclusions: Two 0.454%w/w SnF2 dentifrices have been shown to be effective in reducing DP. However, the ExpSnF2 was significantly more effective in 2-4 day in vitro models, with/without ACC. This difference may translate to a superior clinical benefit for patients.
Methods: HC1&2 involving 48hr HC with/without ACC, respectively, and HC3 involved 4 day HC with ACC. Test Dentifrices were an Experimental Dentifrice (ExpSnF2) and Sensodyne® Repair and Protect (SRP). Human dentine disks (n=10/dentifrice) with patent tubules were placed into a split chamber device connected to an Earle’s solution fluid reservoir. Hydrostatic pressure (1psi) was applied to the underside of the dentine disk and measurement of baseline flow rate recorded. The dentine was treated with dentifrice, the reduced flow rate measured and flow rate percentage reduction from baseline calculated. The dentine and chamber were stored in Artificial Saliva at 37oC for 24hrs post-treatment, and Δ% calculated per treatment. ACC was performed with dietary acid immediately prior to the final measurement for the HC2, and before and after the final treatment in HC3.
Results: Statistical analysis of the results showed that both dentifrices reduced Dentine Permeability (DP) effectively. Treatment differences for Δ% (dΔ%= ExpSnF2(Δ%)-SRP(Δ%)) in each study were: HC1 dΔ%[Initial=22.04%, 24hrs=54.75%, 48hrs=49.39%]; HC2 dΔ%[Initial: 20.37%, 24hrs: 20.73%, 48hrs: 36.99%); HC3 dΔ%[Initial: 23.07%, Day 1: 17.75%, Day 2: 21.91%, Day 3: 19.05%, Day 4: 30.22%].The ExpSnF2 reduced DP to a greater extent than SRP with statistically significant reductions in HC1, 2 and 3 over all time points (p<0.05).
Conclusions: Two 0.454%w/w SnF2 dentifrices have been shown to be effective in reducing DP. However, the ExpSnF2 was significantly more effective in 2-4 day in vitro models, with/without ACC. This difference may translate to a superior clinical benefit for patients.