IADR Abstract Archives
Effect of Material and Storage Temperature on Sealant Microleakage
Objectives: To investigate how material type and storage temperature affect the marginal microleakage of different sealant materials. This study is part of a larger analysis that aims to establish recommendations for management of materials in settings where cooling systems may not be accessible (i.e., extreme climate conditions in resource-limited countries).
Methods: Ninety extracted human teeth were obtained. Samples were randomly assigned into 9 groups (n=10). Three sealants were tested: GC Fugi® Triage GC America (GC), Embrace™ WetBond™ Pulpdent (EW) and Voco Grandio Seal Voco (VG). Materials were placed in an incubator and heated for 72 hours at either 24°C, 40°C or 52°C. All materials were applied according to manufacturer’s instructions. Completed restorations were thermocycled for 500 cycles with a dwell time of 30 seconds between 5°C and 55°C. Samples were immersed in 2% methylene blue dye for 8 hours, embedded in acrylic resin, sectioned bucco-lingually to expose 4 surfaces, and evaluated under stereomicroscope (Olympus, SZX16). A dye-penetration-to-sealant (DS) score was used: 0=0% DS, 1=1-50% DS, 2=51-100% DS, 3= >100% DS (fissure penetration). Counts and percentages were calculated. Statistical significance was assessed via Kruskal-Wallis tests (p < 0.05) for separate comparisons of materials and temperatures; Dunn’s test with Bonferroni correction (p < 0.017) was used for post-hoc comparisons.
Results: Table 1 displays counts and percentages of microleakage scores. Table 2 displays p-values of comparisons between materials for each temperature. Table 3 displays p-values of comparisons between temperatures for each material.
Conclusions: At 24°C, material GC exhibited significantly greater microleakage than EW and VG. At 40°C, material VG exhibited significantly less microleakage than GC. At 52°C, material GC exhibited significantly less microleakage than EW and VG.
Methods: Ninety extracted human teeth were obtained. Samples were randomly assigned into 9 groups (n=10). Three sealants were tested: GC Fugi® Triage GC America (GC), Embrace™ WetBond™ Pulpdent (EW) and Voco Grandio Seal Voco (VG). Materials were placed in an incubator and heated for 72 hours at either 24°C, 40°C or 52°C. All materials were applied according to manufacturer’s instructions. Completed restorations were thermocycled for 500 cycles with a dwell time of 30 seconds between 5°C and 55°C. Samples were immersed in 2% methylene blue dye for 8 hours, embedded in acrylic resin, sectioned bucco-lingually to expose 4 surfaces, and evaluated under stereomicroscope (Olympus, SZX16). A dye-penetration-to-sealant (DS) score was used: 0=0% DS, 1=1-50% DS, 2=51-100% DS, 3= >100% DS (fissure penetration). Counts and percentages were calculated. Statistical significance was assessed via Kruskal-Wallis tests (p < 0.05) for separate comparisons of materials and temperatures; Dunn’s test with Bonferroni correction (p < 0.017) was used for post-hoc comparisons.
Results: Table 1 displays counts and percentages of microleakage scores. Table 2 displays p-values of comparisons between materials for each temperature. Table 3 displays p-values of comparisons between temperatures for each material.
Conclusions: At 24°C, material GC exhibited significantly greater microleakage than EW and VG. At 40°C, material VG exhibited significantly less microleakage than GC. At 52°C, material GC exhibited significantly less microleakage than EW and VG.
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