Effect of Three Hemostatic Agents on Microleakage of Class V Composite Resin Restorations to Caries-Affected Like Dentin Using Self-Etch Adhesive

Objectives: One way to control bleeding in order to acheive a durable bond with less leakage, is the use of hemostatic agents. Given the conservative approach to remain the affected dentin and using self-etch adhesives, this study was done with the aim of investigation of three hemostatic agents on the microleakage of class V composite resin restorations to caries-affected like dentin.
Methods: In this experimental study, 96 class V non-beveled cavities were prepared on the buccal and lingual surfaces of 48 healthy human third molars, 1mm below the CEJ (Cemento Enamel Junction) in cervical margin with the occlusogingival size of 2mm and mesiodistal dimension of 3mm and a depth of 1.5mm. Half of cavities were exposed to mineralization/ demineralization cycles. Each group was divided into 4 subgroups (n=12). Group 1 and 5 were the control groups. In group 2 and 6 Viscostat, for groups 3 and 7, Viscostat Clear and for groups 4 and 8 TCA was used. Then cavities were filled with Clearfill SE Bond and APX composite resin. After 1000 thermocycling and immersion in 2% fuchsin for 24 hours, samples were sectioned buccolingually. Degree of dye penetration was evaluated with microscope with magnification of 56. Results are evaluated based on Kruskal-Wallis and Mann-Whitney tests using SPSS software (a=0.05).
Results: Results showed significant difference between mean of the leakage in different groups (p-value is 0.047). There was significant difference between mean of the dentin leakage in normal and caries-affected like dentin groups (p-value is 0.014). Dentin leakage in group 3 was significantly higher than groups 4,5,6,7 and 8.
Conclusions: In this study, Viscostat Clear led to the highest dentin leakage and TCA led to lowest enamel leakage. Dentin leakage was significantly higher in normal dentin substrate than caries-affected like dentin.