IADR Abstract Archives
Bone Matrix Protein Extract-PEGDA Hydrogel Scaffold for Dental Bone Regeneration
Objectives: Bone defects after tooth extraction present limitations in the restorative treatment of patients. In this context, hydrogels are of great interest as regenerative materials due to their similarity to tissue extracellular matrix (ECM). Proteins extracted from bone have been shown to stimulate bone host cells. However, their fast degradation and insufficient mechanical strength are obstacles for their application in bone regeneration. Therefore, the purpose of this study was to evaluate, optimize and characterize a blend of bone extracellular matrix proteins, extracted from porcine bone, and polyethylene glycol diacrylate (PEGDA) to fabricate an injectable hydrogel that can be used as a a scaffold for bone regeneration/ridge preservation.
Methods: Bone fragments extracted from fresh pig heads were segmentented, pulverized, demineralized in 0.5N HCl and defatted in Methanol-Chloroform (1:1 v/v) to then be digested with 1% w/v pepsin. The resulting product named as Bone Matrix Protein Extract (BMPE) was characterized by electrophoresis and mass spectrometry then blended with PEGDA to optimize the concentration. Crosslinking time by redox polymerization (CLT), compresive strengh (CS), volume expansion (VE), mass loss (ML) and cell proliferation were assessed in the hybrid hydrogels and injectable form was tested (n=5, statistically analyzed ANOVA, Bonferroni, *p<0.05).
Results: The resulting and optimized 0.1% w/v BMPE and 20% w/v PEGDA injectable hybrid hydrogel showed a favorable CLT (90s), a high CS (500Mpa), a controlled VE and ML (1mm3; 8%), cytocompatibility and an interesting porous micro-architecture as shown by scanning electron microscopy (SEM). In addition, injectable form was used providing a convenient and effective system.
Conclusions: Optimized BMPE/PEGDA hydrogels blend offer favorable physicochemical properties and can be delivered in injectable form making the scaffold suitable for the development of new biomaterial for bone tissue regeneration.
Methods: Bone fragments extracted from fresh pig heads were segmentented, pulverized, demineralized in 0.5N HCl and defatted in Methanol-Chloroform (1:1 v/v) to then be digested with 1% w/v pepsin. The resulting product named as Bone Matrix Protein Extract (BMPE) was characterized by electrophoresis and mass spectrometry then blended with PEGDA to optimize the concentration. Crosslinking time by redox polymerization (CLT), compresive strengh (CS), volume expansion (VE), mass loss (ML) and cell proliferation were assessed in the hybrid hydrogels and injectable form was tested (n=5, statistically analyzed ANOVA, Bonferroni, *p<0.05).
Results: The resulting and optimized 0.1% w/v BMPE and 20% w/v PEGDA injectable hybrid hydrogel showed a favorable CLT (90s), a high CS (500Mpa), a controlled VE and ML (1mm3; 8%), cytocompatibility and an interesting porous micro-architecture as shown by scanning electron microscopy (SEM). In addition, injectable form was used providing a convenient and effective system.
Conclusions: Optimized BMPE/PEGDA hydrogels blend offer favorable physicochemical properties and can be delivered in injectable form making the scaffold suitable for the development of new biomaterial for bone tissue regeneration.