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Low Tntensity-ultrasound Combined With Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats

Objectives: This study aims to evaluate the efficacy of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on experimental periodontal disease in rats.
Methods: Periodontal disease was induced by submerging ligatures at the first maxillary molar subgingival region in forty-eight male SD rats. After 30 days, the ligatures were removed. The rats were randomly allocated into four groups; the experimental SDT group was treated through hypodermic injection of 40 μg/mL HMME and 3 W/cm2 low-intensity ultrasound irradiation (1 MHz, 600 s). Those in control groups were received to 40 μg/mL HMME alone (control 1 group) or to 3 W/cm2 ultrasound irradiation alone (control 2 group) or subjected neither HMME nor ultrasound (control 3 group). After 10 days treatment, all rats were euthanized, the maxilla was obtained for histological examination and the alveolar bone level was evaluated by histometric analysis.
Results: The control groups showed more bone loss (P<0.05) after 10 days treatment than the SDT group. There is no significant difference among the control groups (P>0.05).
Conclusions: HMME mediated-SDT was an effective therapy of experimental periodontal tissue in rats.
Division: IADR/AADR/CADR General Session
Meeting: 2017 IADR/AADR/CADR General Session (San Francisco, California)
Location: San Francisco, California
Year: 2017
Final Presentation ID: 4033
Abstract Category|Abstract Category(s): Periodontal Research-Therapy
Authors
  • Zhuang, Deshu  ( Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China , Harbin , China )
  • Bi, Liangjia  ( Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China , Harbin , China )
    • Support Funding Agency/Grant Number: Natural Science Foundation Of China [grant number 81670994] and the Joint Specialized Research Fund for the Doctoral Program of Higher Education, MOE of China [grant number 20132307110018]
    Financial Interest Disclosure: NONE
    SESSION INFORMATION
    Poster Session
    Novel Approaches to Treat Periodontal Diseases
    Saturday, 03/25/2017 , 03:45PM - 05:00PM
    IMAGES
    Figure 1 Schematic diagram of the SDT system.<br /> Notes: (A) Schematic diagram of the ultrasound system. (B) Photograph in vivo of the injection site of HMME. (C) Photograph in vivo of the transducer in action.<br />
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    Figure 2 Photomicrographs of bone tissue in the alveolar bone area between the first and second maxillary molars with induced periodontitis.<br /> Notes: (A1) SDT group at 10 days. (A2) Areas of thick bone trabecula without signs of resorption in the SDT group at 10 days. (B1) Control 1 group at 10 days. (B2) Areas of bone resorption with thin bone trabecula and disorganized connective tissue in the Control 1 group at 10 days. (C1) Control 2 group at 10 days. (C2) Areas of bone resorption with thin bone trabecula and disorganized connective tissue in the Control 2 group at 10 days. (D1) Control 3 group at 10 days. (D2) Areas of bone resorption with thin bone trabecula and disorganized connective tissue in the Control 3 group at 10 days. Hematoxylin and eosin staining; original magnification for A, B, C, and D was ×4; A2, B2, C2, D2 was ×40.
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    Figure 3 Mean ± standard deviation of the data of the distance between the CEJ and ABC (mm) of the alveolar bone area between the first and second maxillary molars in each group.<br /> Notes: <sup>*</sup>Compared with the control 3 (P<0.05; ANOVA). <sup>#</sup>Compared with the control 3 (P>0.05; ANOVA). <sup>†</sup>Compared with the control 3 (P=0.068; ANOVA). <sup>†</sup>Compared with the control 1 (P>0.05; ANOVA). <br /> Abbreviations: ANOVA, analysis of variance.<br />
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