Influence of Crude Drugs Combination Resin on Angiogenesis in vitro

Objectives: The purpose of this study was to determine the influence of crude drugs on the dynamic viscoelasticity of soft acrylic resin and angiogenesis in vitro.
Methods: Two poly (ethyl methacrylate) (Negami Chemical Industrial Co.) with different molecular weight and particle size were used as powder form. The combination of 95 wt% Citroflex A-4 (ATBC) (Tokyo Chemical Industry Co.) and 5 wt% ethyl alcohol was used as the liquid phase. Two kinds of crude drugs (Astragalus membranaceus Bunge (Huangqi) and Salvia miltiorrhiza Bunge (Danshen)) were used in this study. The dynamic viscoelasticity of each specimen was measured after 0, 1, 3, 7 and 14 days of immersion at 37°C distilled water using DMA. The CellPlayer angiogenesis PrimeKit assay (ESSEN Bioscience; Human Umbilical Vein Endothelial Cells with Normal Human Dermal Fibroblasts) were used to angiogenesis test. After immersed each material to the culture medium in a CO₂ incubator, the culture was made therein for 3 days. After 3 days, the length, and area of new capillary formation were measured using an angiogenesis image analyzer software (ESSEN Bioscience). All data were analyzed by ANOVA at a 0.05 level of significance.
Results: The significant difference was found among the materials in the dynamic viscoelasticity. About the angiogenesis, the significant difference was found among the different content and type of crude drugs in the length and area of new capillary (p<0.05, one-way ANOVA). The length and area of new capillary in the low content (1 wt%) of crude drugs materials was the highest than control group and high content (5 wt% and 10 wt%) of crude drugs materials.
Conclusions: The results suggest that the add the crude drugs with low content to the powder of a soft acrylic resin can promote the angiogenesis of human tissue.