NFKB1 Polymorphisms and Prognosis of OSCC Patients

Objectives: The aims of this study were to determine the roles of NFKB1 -94ATTG Ins/Del polymorphisms in prognosis of oral squamous cell carcinoma (OSCC) patients.
Methods: OSCC patients who had pathologists and histologically confirmation were recruited from the department of Oral Maxillofacial Surgery, Kaohsiung Medical University Hospital betwwen 2003 and 2009. All experimental protocols were approved by KMUH IRB. A total of 5 ml of peripheral blood sample was collected for DNA extraction. The determination of genotypes of NFKB1 gene were completed using the PCR-RFLP method. The demographic chacterstics and histories of three substances' usage were collected by a standardized structural questionnaire. The results of inital diagnosis, clinical and histological characteristics, clinical treament, recurrence, survival or death, and metastasis were collected from medical records charting. The kaplan-meier survival curve, log-rank test, and cox proportional hazards model analysis were used to test the associations.
Results: A total of 295 OSCC patients were recruited into this study. No associations were found between cancer stage, tumor size, metastasis, recurence, and NFκB1 SNPs. The frequencies of NFκB1 -94ATTG Ins/Ins+Ins/Del genotypes were significantly higher in OSCC patients with the treatment of surgery alone. In the nine-year follow-up medical records, survival analysis showed that NFκB1 SNPs were not correlated with low disease-free survival or low overall survival. Stratification analysis with different cancer treatments showed that NFκB1 -94ATTG Ins/Del SNPs were associated with the lower disease-free survival rate and overall survival in OSCC patients treated with surgery alone.
Conclusions: These results suggest that NFKB1 SNPs may not correlate with the survival of OSCC patients. However, NFκB1 -94ATTG Ins/Del polymorphisms can modify the disease-free survival rate and overall survival in OSCC patients treated with surgery alone.