The Collagen Membrane Containing With Osteogenic Protein-1 Facilitates Bone Regeneration

Objectives: Regenerative surgery involving the use of bioabsorbable collagen membranes (BCM) has proven to be an effective method for periodontal and bone regeneration and is widely used for the treatment of periodontal and bone defects for proper dental implants placement. Osteogenic protein-1 (OP-1) are generally accepted to be the most osteoinductive factors and are currently used clinically to induce new bone formation in the field of orthopedics or dentistry. We have devised the regeneration device that is combination of BCM containing with OP-1 (BCM/OP-1) for bone regeneration. The main purpose of this study is to evaluate the effects of BCM/OP-1 on bone healing in a rat mandibular bone defect model in vivo.
Methods: Mandibular defects (4.0 mm in diameter) were created, and the following treatments were applied: (1) no treatment; (2) BCM alone; (3) CM containing a low dose (0.5 μg) of OP-1 (L-OP-1); and (4) CM containing a high dose (2.0 μg) of OP-1 (H-OP-1). The rats were scanned at 0, 2, 4, 6, and 8 weeks postoperatively using micro computed tomography (micro-CT) to obtain three-dimensional reconstructed visualizations using volume rendering. The newly formed bone was evaluated by micro-CT and histological analysis at each weeks after surgery.
Results: The results of this studies revealed that BCM/OP-1 significantly increased and accelerated bone regeneration compared to BCM alone or control in the quantitative and qualitative micro-CT analysis for volume of new bone formation, bone density, and the percentage of new bone area. The enhancements of bone regeneration in OP-1-treated groups were dose-dependent.
Conclusions: These results suggest that BCM/OP-1 possesses osteoinductive properties which are sufficient for bone regeneration. Hence, the use of this combined device for osteogenesis is a unique concept that may result in more safe and predictable bone regenerative outcomes in the future.