IADR Abstract Archives

Variability of Salivary Oxidative Stress Markers in Healthy Subjects

Objectives: Little data exist regarding normal variations for salivary oxidative stress (OS) markers in healthy population. The aim of this study was to determine the normal intra- and inter-day variations of salivary OS markers in healthy adult individuals.
Methods: Unstimulated saliva from 15 healthy adults was collected twice daily (7:00 AM and 5:00 PM) on three consecutive days. Salivary levels of OS markers, including glutathione peroxidase (GPX), superoxide dismutase (SOD), total antioxidant capacity (TAC), 8-hydroxydeoxyguanosine (8-0HdG) and uric acid (UA) were determined.
Results: Concentrations of most measured markers did not differ significantly during the day; only SOD showed significant intra-day variations, with significantly higher values in the morning compared to the afternoon measurements (F=12.72, p=0.003). A within subjects MANOVA did not show the significant influence of “sampling day” as a factor affecting the variability of salivary OS markers (P>0.05). The highest intraclass correlations (ICC’s) were obtained for UA and 8-0HdG (0.871 and 0.858 respectively).
Conclusions: Salivary OS markers can be measured reproducibly over time, since no significant inter-day variability for all measured markers was observed. Moreover, concentrations of most measured markers did not differ significantly during the day. Only SOD showed significant intra-day variations. The excellent reliability obtained for UA and 8-0HdG indicates that those markers could potentially be more specific in terms of distinguishing patient population from the healthy controls.
IADR/AADR/CADR General Session
2017 IADR/AADR/CADR General Session (San Francisco, California)
San Francisco, California
2017
1648
  • Alajbeg, Iva  ( School of Dental Medicine , Zagreb Zagreb , Croatia )
  • Rogić, Dunja  ( Universtiy Hospital Center Zagreb , Zagreb Zagreb , N/A = Not Applicable , Croatia )
  • Illeš, Davor  ( School of Dental Medicine , Zagreb Zagreb , Croatia )
  • Vuletic, Lea  ( School of Dental Medicine, University of Zagreb, Croatia , Zagreb Zagreb , N/A = Not Applicable , Croatia )
  • Andabak Rogulj, Ana  ( School of Dental Medicine, University of Zagreb, Croatia , Zagreb Zagreb , N/A = Not Applicable , Croatia )
  • Lapić, Ivana  ( Universtiy Hospital Center Zagreb , Zagreb Zagreb , N/A = Not Applicable , Croatia )
  • Knezović Zlatarić, Dubravka  ( School of Dental Medicine , Zagreb Zagreb , Croatia )
  • Badel, Tomislav  ( School of Dental Medicine , Zagreb Zagreb , Croatia )
  • Alajbeg, Ivan  ( School of Dental Medicine, University of Zagreb, Croatia , Zagreb Zagreb , N/A = Not Applicable , Croatia )
  • This work has been fully supported by Croatian Science Foundation under the project (IP-2014-09-3070).
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    Poster Session
    Salivary Diagnostic and Salivary Composition I
    Thursday, 03/23/2017 , 03:45PM - 05:00PM